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• W2068970778 abstract "The adaptive immune system recognizes billions of unique antigens using highly variable T-cell receptors. The αβ T-cell receptor repertoire includes an estimated 106 different rearranged β chains per individual. This paper describes a novel micro-array based method that monitors the β chain repertoire with a resolution of a single T-cell clone. These T-arrays are quantitative and detect T-cell clones at a frequency of less than one T cell in a million, which is 2 logs more sensitive than spectratyping (immunoscope), the current standard in repertoire analysis. Using T-arrays we detected CMV-specific CD4+ and CD8+ T-cell clones that expanded early after viral antigen stimulation in vitro and in vivo. This approach will be useful in monitoring individual T-cell clones in diverse experimental settings, and in identification of T-cell clones associated with infectious disease, autoimmune disease and cancer." @default.
• W2068970778 created "2016-06-24" @default.
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• W2068970778 date "2006-12-20" @default.
• W2068970778 modified "2023-10-18" @default.
• W2068970778 title "Monitoring the T-Cell Receptor Repertoire at Single-Clone Resolution" @default.
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• W2068970778 doi "https://doi.org/10.1371/journal.pone.0000055" @default.
• W2068970778 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1762342" @default.
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