Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068996919> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2068996919 endingPage "152" @default.
- W2068996919 startingPage "148" @default.
- W2068996919 abstract "Identification of human T-helper cell subsets is possible by measurement of intracellular cytokines after coincubation of lymphocytes with phorbol myristate acetate (PMA), calcium ionophore, and brefeldin A for up to 20 h. However, exposure to PMA leads to internalization of membrane CD4 and to loss of resolution of the CD4+ cells. Detection of CD3+CD8- cells or preselection of CD4+ cells prior to stimulation is more cumbersome than direct measurement of CD4+ cells. We report the use of the Leu3a/Leu3b multiclone for the accurate determination of CD4 cells after PMA stimulation.Peripheral blood lymphocytes were isolated from healthy normal donors and the proportion of CD3+ / CD4+ T cells was determined by flow cytometry before and after incubation with PMA, calcium ionophore, and brefeldin A for 20 h using a variety of anti-CD4 monoclonal antibodies.The Leu3a/3b multiclone reagent was the only anti-CD4 monoclonal antibody capable of resolving more than 98% of the initial CD4+ events after incubation with PMA.The higher signal-to-noise ratio associated with Leu3a3b reagent, compared with other CD4-specific antibodies available, allows the direct and accurate identification of the CD4 subset even after PMA treatment of cells." @default.
- W2068996919 created "2016-06-24" @default.
- W2068996919 creator A5008546894 @default.
- W2068996919 creator A5012573548 @default.
- W2068996919 creator A5018399833 @default.
- W2068996919 creator A5059990280 @default.
- W2068996919 creator A5087375137 @default.
- W2068996919 date "2001-01-01" @default.
- W2068996919 modified "2023-10-18" @default.
- W2068996919 title "Use of Leu3a/3b for the accurate determination of CD4 subsets for measurement of intracellular cytokines" @default.
- W2068996919 cites W1968999883 @default.
- W2068996919 cites W1982778833 @default.
- W2068996919 cites W2028055683 @default.
- W2068996919 cites W2055625331 @default.
- W2068996919 cites W2055669282 @default.
- W2068996919 cites W2057631395 @default.
- W2068996919 doi "https://doi.org/10.1002/1097-0320(20010601)44:2<148::aid-cyto1094>3.0.co;2-6" @default.
- W2068996919 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11378866" @default.
- W2068996919 hasPublicationYear "2001" @default.
- W2068996919 type Work @default.
- W2068996919 sameAs 2068996919 @default.
- W2068996919 citedByCount "12" @default.
- W2068996919 countsByYear W20689969192012 @default.
- W2068996919 countsByYear W20689969192013 @default.
- W2068996919 countsByYear W20689969192014 @default.
- W2068996919 countsByYear W20689969192020 @default.
- W2068996919 crossrefType "journal-article" @default.
- W2068996919 hasAuthorship W2068996919A5008546894 @default.
- W2068996919 hasAuthorship W2068996919A5012573548 @default.
- W2068996919 hasAuthorship W2068996919A5018399833 @default.
- W2068996919 hasAuthorship W2068996919A5059990280 @default.
- W2068996919 hasAuthorship W2068996919A5087375137 @default.
- W2068996919 hasBestOaLocation W20689969191 @default.
- W2068996919 hasConcept C114684123 @default.
- W2068996919 hasConcept C119062480 @default.
- W2068996919 hasConcept C147483822 @default.
- W2068996919 hasConcept C1491633281 @default.
- W2068996919 hasConcept C153911025 @default.
- W2068996919 hasConcept C159654299 @default.
- W2068996919 hasConcept C167672396 @default.
- W2068996919 hasConcept C178790620 @default.
- W2068996919 hasConcept C181080969 @default.
- W2068996919 hasConcept C185592680 @default.
- W2068996919 hasConcept C203014093 @default.
- W2068996919 hasConcept C2776989580 @default.
- W2068996919 hasConcept C2779014961 @default.
- W2068996919 hasConcept C2781373781 @default.
- W2068996919 hasConcept C519063684 @default.
- W2068996919 hasConcept C542903549 @default.
- W2068996919 hasConcept C553184892 @default.
- W2068996919 hasConcept C55493867 @default.
- W2068996919 hasConcept C79879829 @default.
- W2068996919 hasConcept C86803240 @default.
- W2068996919 hasConceptScore W2068996919C114684123 @default.
- W2068996919 hasConceptScore W2068996919C119062480 @default.
- W2068996919 hasConceptScore W2068996919C147483822 @default.
- W2068996919 hasConceptScore W2068996919C1491633281 @default.
- W2068996919 hasConceptScore W2068996919C153911025 @default.
- W2068996919 hasConceptScore W2068996919C159654299 @default.
- W2068996919 hasConceptScore W2068996919C167672396 @default.
- W2068996919 hasConceptScore W2068996919C178790620 @default.
- W2068996919 hasConceptScore W2068996919C181080969 @default.
- W2068996919 hasConceptScore W2068996919C185592680 @default.
- W2068996919 hasConceptScore W2068996919C203014093 @default.
- W2068996919 hasConceptScore W2068996919C2776989580 @default.
- W2068996919 hasConceptScore W2068996919C2779014961 @default.
- W2068996919 hasConceptScore W2068996919C2781373781 @default.
- W2068996919 hasConceptScore W2068996919C519063684 @default.
- W2068996919 hasConceptScore W2068996919C542903549 @default.
- W2068996919 hasConceptScore W2068996919C553184892 @default.
- W2068996919 hasConceptScore W2068996919C55493867 @default.
- W2068996919 hasConceptScore W2068996919C79879829 @default.
- W2068996919 hasConceptScore W2068996919C86803240 @default.
- W2068996919 hasIssue "2" @default.
- W2068996919 hasLocation W20689969191 @default.
- W2068996919 hasLocation W20689969192 @default.
- W2068996919 hasOpenAccess W2068996919 @default.
- W2068996919 hasPrimaryLocation W20689969191 @default.
- W2068996919 hasRelatedWork W193412453 @default.
- W2068996919 hasRelatedWork W2015506378 @default.
- W2068996919 hasRelatedWork W2038339438 @default.
- W2068996919 hasRelatedWork W2068996919 @default.
- W2068996919 hasRelatedWork W2083679349 @default.
- W2068996919 hasRelatedWork W2143087365 @default.
- W2068996919 hasRelatedWork W2144018395 @default.
- W2068996919 hasRelatedWork W2409777197 @default.
- W2068996919 hasRelatedWork W2420935970 @default.
- W2068996919 hasRelatedWork W2441422483 @default.
- W2068996919 hasVolume "44" @default.
- W2068996919 isParatext "false" @default.
- W2068996919 isRetracted "false" @default.
- W2068996919 magId "2068996919" @default.
- W2068996919 workType "article" @default.