FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069081503> ?p ?o ?g. }

• W2069081503 endingPage "e69041" @default.
• W2069081503 startingPage "e69041" @default.
• W2069081503 abstract "The C2 domain of PKCα possesses two different binding sites, one for Ca(2+) and phosphatidylserine and a second one that binds PIP2 with very high affinity. The enzymatic activity of PKCα was studied by activating it with large unilamellar lipid vesicles, varying the concentration of Ca(2+) and the contents of dioleylglycerol (DOG), phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphadidylserine (POPS) in these model membranes. The results showed that PIP2 increased the Vmax of PKCα and, when the PIP2 concentration was 5 mol% of the total lipid in the membrane, the addition of 2 mol% of DOG did not increase the activity. In addition PIP2 decreases K0.5 of Ca(2+) more than 3-fold, that of DOG almost 5-fold and that of POPS by a half. The K0.5 values of PIP2 amounted to only 0.11 µM in the presence of DOG and 0.39 in its absence, which is within the expected physiological range for the inner monolayer of a mammalian plasma membrane. As a consequence, PKCα may be expected to operate near its maximum capacity even in the absence of a cell signal producing diacylglycerol. Nevertheless, we have shown that the presence of DOG may also help, since the K0.5 for PIP2 notably decreases in its presence. Taken together, these results underline the great importance of PIP2 in the activation of PKCα and demonstrate that in its presence, the most important cell signal for triggering the activity of this enzyme is the increase in the concentration of cytoplasmic Ca(2+)." @default.
• W2069081503 created "2016-06-24" @default.
• W2069081503 creator A5035305615 @default.
• W2069081503 creator A5056099299 @default.
• W2069081503 creator A5063168291 @default.
• W2069081503 creator A5090329327 @default.
• W2069081503 date "2013-07-10" @default.
• W2069081503 modified "2023-10-17" @default.
• W2069081503 title "Phosphatidylinositol 4,5-Bisphosphate Decreases the Concentration of Ca2+, Phosphatidylserine and Diacylglycerol Required for Protein Kinase C α to Reach Maximum Activity" @default.
• W2069081503 cites W1420109459 @default.
• W2069081503 cites W1486602990 @default.
• W2069081503 cites W1486711418 @default.
• W2069081503 cites W1496062184 @default.
• W2069081503 cites W1576671418 @default.
• W2069081503 cites W1588018069 @default.
• W2069081503 cites W1595133660 @default.
• W2069081503 cites W1603607026 @default.
• W2069081503 cites W1676701054 @default.
• W2069081503 cites W1971109022 @default.
• W2069081503 cites W1973432756 @default.
• W2069081503 cites W1974606305 @default.
• W2069081503 cites W1977239487 @default.
• W2069081503 cites W1977986845 @default.
• W2069081503 cites W1988364063 @default.
• W2069081503 cites W1991585139 @default.
• W2069081503 cites W1993171928 @default.
• W2069081503 cites W1993973400 @default.
• W2069081503 cites W1994119115 @default.
• W2069081503 cites W1999226878 @default.
• W2069081503 cites W2018878700 @default.
• W2069081503 cites W2027942301 @default.
• W2069081503 cites W2029583669 @default.
• W2069081503 cites W2034266881 @default.
• W2069081503 cites W2041196045 @default.
• W2069081503 cites W2050102433 @default.
• W2069081503 cites W2050524093 @default.
• W2069081503 cites W2052108557 @default.
• W2069081503 cites W2058143246 @default.
• W2069081503 cites W2059335098 @default.
• W2069081503 cites W2060982278 @default.
• W2069081503 cites W2066855988 @default.
• W2069081503 cites W2067234382 @default.
• W2069081503 cites W2070884121 @default.
• W2069081503 cites W2072238788 @default.
• W2069081503 cites W2072360439 @default.
• W2069081503 cites W2077520727 @default.
• W2069081503 cites W2078901357 @default.
• W2069081503 cites W2079628880 @default.
• W2069081503 cites W2082412503 @default.
• W2069081503 cites W2083432361 @default.
• W2069081503 cites W2090374527 @default.
• W2069081503 cites W2091884933 @default.
• W2069081503 cites W2096964838 @default.
• W2069081503 cites W2097331211 @default.
• W2069081503 cites W2099133435 @default.
• W2069081503 cites W2122470288 @default.
• W2069081503 cites W2134283668 @default.
• W2069081503 cites W2163888620 @default.
• W2069081503 cites W2165578823 @default.
• W2069081503 cites W2409941212 @default.
• W2069081503 cites W4322700664 @default.
• W2069081503 cites W4360754768 @default.
• W2069081503 doi "https://doi.org/10.1371/journal.pone.0069041" @default.
• W2069081503 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3707892" @default.
• W2069081503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23874859" @default.
• W2069081503 hasPublicationYear "2013" @default.
• W2069081503 type Work @default.
• W2069081503 sameAs 2069081503 @default.
• W2069081503 citedByCount "12" @default.
• W2069081503 countsByYear W20690815032013 @default.
• W2069081503 countsByYear W20690815032014 @default.
• W2069081503 countsByYear W20690815032016 @default.
• W2069081503 countsByYear W20690815032017 @default.
• W2069081503 countsByYear W20690815032021 @default.
• W2069081503 countsByYear W20690815032022 @default.
• W2069081503 crossrefType "journal-article" @default.
• W2069081503 hasAuthorship W2069081503A5035305615 @default.
• W2069081503 hasAuthorship W2069081503A5056099299 @default.
• W2069081503 hasAuthorship W2069081503A5063168291 @default.
• W2069081503 hasAuthorship W2069081503A5090329327 @default.
• W2069081503 hasBestOaLocation W20690815031 @default.
• W2069081503 hasConcept C12554922 @default.
• W2069081503 hasConcept C130316041 @default.
• W2069081503 hasConcept C181199279 @default.
• W2069081503 hasConcept C184235292 @default.
• W2069081503 hasConcept C185592680 @default.
• W2069081503 hasConcept C190062978 @default.
• W2069081503 hasConcept C195794163 @default.
• W2069081503 hasConcept C2777853560 @default.
• W2069081503 hasConcept C2778918659 @default.
• W2069081503 hasConcept C2779637612 @default.
• W2069081503 hasConcept C2780610907 @default.
• W2069081503 hasConcept C36020004 @default.
• W2069081503 hasConcept C41625074 @default.
• W2069081503 hasConcept C49658373 @default.
• W2069081503 hasConcept C55493867 @default.
• W2069081503 hasConcept C86803240 @default.
• W2069081503 hasConcept C95444343 @default.

• next