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- W2069088488 abstract "We attempted to establish a system to generate monoclonal antibodies (mAbs) recognizing CD antigens on lymphocytes of domestic animals using the expressed CD antigens by a baculovirus expression system. For this purpose, we selected feline CD4 (fCD4) antigen and expressed it in an insect cell line (Sf9 cells). To obtain mAbs, BALB/c mice were immunized with feline peripheral blood mononuclear cells (fPBMCs) or Sf9 cells expressing the fCD4 (Sf-fCD4 cells), and then hybridomas secreting antibodies were screened by indirect immunofluorescence assay against the opposite antigens of Sf-fCD4 cells or fPBMCs, respectively. Five mAbs recognizing the fCD4 were obtained in total. The system established here might be useful to obtain mAbs recognizing CD antigens of domestic animals." @default.
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- W2069088488 date "1997-01-01" @default.
- W2069088488 modified "2023-10-05" @default.
- W2069088488 title "Generation of Monoclonal Antibodies against a Feline CD Antigen (CD4) Expressed by a Recombinant Baculovirus." @default.
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- W2069088488 doi "https://doi.org/10.1292/jvms.59.467" @default.
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