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- W2069088839 abstract "In order to reach their final destination, periplasmic and outer membrane proteins have to pass the cytoplasmic membrane of Escherichia coli cells. To study the transport of PhoE protein, we developed an in vitro transcription-translation and translocation system. In this in vitro system, the protein is synthesized as a larger precursor, which can be processed by purified leader peptidase. The precursor can be translocated into inverted inner membrane vesicles as judged by the protection against externally added protease. Only part of the translocated protein is in the processed mature form. Translocation can occur posttranslationally and requires both ATP and the protonmotive force for an optimal process. Upon incubation of vesicles with mature PhoE protein or precursor PhoE in the absence of ATP, the proteins are bound to the vesicles, but they are not translocated, since they are still sensitive to externally added protease." @default.
- W2069088839 created "2016-06-24" @default.
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- W2069088839 creator A5050909498 @default.
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- W2069088839 date "1987-06-01" @default.
- W2069088839 modified "2023-10-17" @default.
- W2069088839 title "Optimal posttranslational translocation of the precursor of PhoE protein across Escherichia coli membrane vesicles requires both ATP and the protonmotive force" @default.
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- W2069088839 doi "https://doi.org/10.1016/0005-2736(87)90278-1" @default.
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