FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069148384> ?p ?o ?g. }

• W2069148384 endingPage "871" @default.
• W2069148384 startingPage "860" @default.
• W2069148384 abstract "A chromosomal fragment containing DNA downstream from mauC was isolated from Paracoccus denitrificans. Sequence analysis of this fragment revealed the presence of four open reading frames, all transcribed in the same direction. The products of the putative genes were found to be highly similar to MauJ, MauG, MauM and MauN of Methylobacterium extorquens AM1. Using these four mau genes, 11 mau genes have been cloned from P. denitrificans to date. The gene order is mauRFBEDACJGMN, which is similar to that in M. extorquens AM1. mauL, present in M. extorquens AM1, seems to be absent in P. denitrificans. MauJ is predicted to be a cytoplasmic protein, and MauG a periplasmic protein. The latter protein contains two putative heme-binding sites, and has some sequence resemblance to the cytochrome c peroxidase from Pseudomonas aeruginosa. MauM is also predicted to be located in the periplasm, but MauN appears to be membrane associated. Both resemble ferredoxin-like proteins and contain four and two motifs, respectively, characteristic for [4Fe-4S] clusters. Inactivation of mauA, mauJ, mauG, mauM and mauN was carried out by introduction of unmarked mutations in the chromosomal copies of these genes. mauA and mauG mutant strains were unable to grow on methylamine. The mauJ mutant strain had an impaired growth rate and showed a lower dye-linked methylamine dehydrogenase (MADH) activity than the parent strain. Mutations in mauM and mauN had no effect on methylamine metabolism. The mauA mutant strain specifically lacked the β subunit of MADH, but the α subunit and amicyanin, the natural electron acceptors of MADH, were still produced. The mauG mutant strain synthesized the α and β subunits of MADH as well as amicyanin. However, no dye-linked MADH activity was found in this mutant strain. In addition, as the wild-type enzyme displays a characteristic fluorescence emission spectrum upon addition of methylamine, this property was lost in the mauG mutant strain. These results clearly show that MauG is essential for the maturation of the β subunit of MADH, presumably via a step in the biosynthesis of tryptophan tryptophylquinone, the cofactor of MADH. The mau gene cluster mauRFBEDACJGMN was cloned on the broad-host vector pEG400. Transfer of this construct to mutant strains which were unable to grow on methylamine fully restored their ability to grow on this compound. A similar result was achieved for the closely related bacterium Thiosphaera pantotropha, which is unable to utilize methylamine as the sole sources of carbon and energy." @default.
• W2069148384 created "2016-06-24" @default.
• W2069148384 creator A5009413314 @default.
• W2069148384 creator A5029742512 @default.
• W2069148384 creator A5046259982 @default.
• W2069148384 creator A5052039097 @default.
• W2069148384 creator A5056969191 @default.
• W2069148384 creator A5070364726 @default.
• W2069148384 creator A5074216951 @default.
• W2069148384 date "2008-06-28" @default.
• W2069148384 modified "2023-09-26" @default.
• W2069148384 title "Mutational Analysis of Mau Genes Involved in Methylamine Metabolism in Paracoccus Denitrificans" @default.
• W2069148384 cites W1516495306 @default.
• W2069148384 cites W1555946545 @default.
• W2069148384 cites W1589880964 @default.
• W2069148384 cites W1663610519 @default.
• W2069148384 cites W1691695228 @default.
• W2069148384 cites W171398818 @default.
• W2069148384 cites W1810520313 @default.
• W2069148384 cites W1866800464 @default.
• W2069148384 cites W1896635935 @default.
• W2069148384 cites W1963752204 @default.
• W2069148384 cites W1967923354 @default.
• W2069148384 cites W1970220520 @default.
• W2069148384 cites W1984649984 @default.
• W2069148384 cites W1987459278 @default.
• W2069148384 cites W2007523504 @default.
• W2069148384 cites W2019841880 @default.
• W2069148384 cites W2020319636 @default.
• W2069148384 cites W2028622989 @default.
• W2069148384 cites W2042606712 @default.
• W2069148384 cites W2042632460 @default.
• W2069148384 cites W2049684911 @default.
• W2069148384 cites W2052207059 @default.
• W2069148384 cites W2055043387 @default.
• W2069148384 cites W2062167275 @default.
• W2069148384 cites W2063450941 @default.
• W2069148384 cites W2070848882 @default.
• W2069148384 cites W2082757999 @default.
• W2069148384 cites W2084597458 @default.
• W2069148384 cites W2095880954 @default.
• W2069148384 cites W2098083368 @default.
• W2069148384 cites W2100339465 @default.
• W2069148384 cites W2100837269 @default.
• W2069148384 cites W2107486700 @default.
• W2069148384 cites W2114671452 @default.
• W2069148384 cites W2133646599 @default.
• W2069148384 cites W2134354689 @default.
• W2069148384 cites W2134515925 @default.
• W2069148384 cites W2142869130 @default.
• W2069148384 cites W2143641357 @default.
• W2069148384 cites W2147447349 @default.
• W2069148384 cites W2161524167 @default.
• W2069148384 cites W2490701537 @default.
• W2069148384 cites W4244698100 @default.
• W2069148384 cites W4251060022 @default.
• W2069148384 cites W4252918532 @default.
• W2069148384 cites W67212647 @default.
• W2069148384 doi "https://doi.org/10.1111/j.1432-1033.1995.0860g.x" @default.
• W2069148384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7601147" @default.
• W2069148384 hasPublicationYear "2008" @default.
• W2069148384 type Work @default.
• W2069148384 sameAs 2069148384 @default.
• W2069148384 citedByCount "14" @default.
• W2069148384 countsByYear W20691483842015 @default.
• W2069148384 countsByYear W20691483842018 @default.
• W2069148384 countsByYear W20691483842020 @default.
• W2069148384 crossrefType "journal-article" @default.
• W2069148384 hasAuthorship W2069148384A5009413314 @default.
• W2069148384 hasAuthorship W2069148384A5029742512 @default.
• W2069148384 hasAuthorship W2069148384A5046259982 @default.
• W2069148384 hasAuthorship W2069148384A5052039097 @default.
• W2069148384 hasAuthorship W2069148384A5056969191 @default.
• W2069148384 hasAuthorship W2069148384A5070364726 @default.
• W2069148384 hasAuthorship W2069148384A5074216951 @default.
• W2069148384 hasBestOaLocation W20691483841 @default.
• W2069148384 hasConcept C104292427 @default.
• W2069148384 hasConcept C104317684 @default.
• W2069148384 hasConcept C134621786 @default.
• W2069148384 hasConcept C143065580 @default.
• W2069148384 hasConcept C153911025 @default.
• W2069148384 hasConcept C181199279 @default.
• W2069148384 hasConcept C201663137 @default.
• W2069148384 hasConcept C2778969155 @default.
• W2069148384 hasConcept C2780640746 @default.
• W2069148384 hasConcept C54355233 @default.
• W2069148384 hasConcept C547475151 @default.
• W2069148384 hasConcept C55493867 @default.
• W2069148384 hasConcept C86803240 @default.
• W2069148384 hasConceptScore W2069148384C104292427 @default.
• W2069148384 hasConceptScore W2069148384C104317684 @default.
• W2069148384 hasConceptScore W2069148384C134621786 @default.
• W2069148384 hasConceptScore W2069148384C143065580 @default.
• W2069148384 hasConceptScore W2069148384C153911025 @default.
• W2069148384 hasConceptScore W2069148384C181199279 @default.
• W2069148384 hasConceptScore W2069148384C201663137 @default.
• W2069148384 hasConceptScore W2069148384C2778969155 @default.
• W2069148384 hasConceptScore W2069148384C2780640746 @default.

• next