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• W2069177031 abstract "We have developed an in vivo micronucleus (MN) test that uses rat skin as the target organ. Sample preparation involves cold-treating the epidermis with trypsin, peeling it off with a fine forceps, treating it in hypotonic solution, and staining it with acridine orange (A.O.). We evaluated the assay using mitomycin C (MMC) and methyl methanesulfonate (MMS) as model clastogens, applying them as single and repeat treatments. Both chemicals induced a significant, dose-dependent increase in MN frequency in basal cells. One treatment per day for 3 days was optimal for MN induction." @default.
• W2069177031 created "2016-06-24" @default.
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• W2069177031 date "1999-07-01" @default.
• W2069177031 modified "2023-09-29" @default.
• W2069177031 title "Study of a rat skin in vivo micronucleus test: data generated by mitomycin C and methyl methanesulfonate" @default.
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• W2069177031 doi "https://doi.org/10.1016/s1383-5718(99)00084-4" @default.
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