Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069181838> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2069181838 endingPage "129" @default.
- W2069181838 startingPage "125" @default.
- W2069181838 abstract "A new micromethod for measuring enzyme-catalyzed reactions was developed. The method involves a number of consecutive direct injections of aliquots of the reaction mixture onto a microbore column and permits the determination of the time dependence of the decrease of substrate or increase of product concentrations. The reactions proceed in a microvial placed in the autosampler, and as the starting volume can be as low as 10 μl, the requirement for the amount of enzyme is very low. The autosampler backed by the liquid chromatographic software allows automation of the analyses including data processing and easy quantitation of the enzymatic reaction(s). The method was applied to a system of two consecutive terminal reactions of tetracycline biosynthesis in Streptomyces aureofaciens, catalyzed by anhydrotetracycline oxygenase and tetracycline dehydrogenase. The usage of a diode-array detector facilitated the quantification of the reactions as the product and substrate could be monitored at their optimal wavelengths." @default.
- W2069181838 created "2016-06-24" @default.
- W2069181838 creator A5020075142 @default.
- W2069181838 creator A5044586504 @default.
- W2069181838 creator A5060128303 @default.
- W2069181838 creator A5061619473 @default.
- W2069181838 creator A5085655903 @default.
- W2069181838 date "1989-08-01" @default.
- W2069181838 modified "2023-10-17" @default.
- W2069181838 title "A direct-injection reversed-phase liquid chromatographic micromethod for studying the kinetics of terminal reactions of tetracycline biosynthesis" @default.
- W2069181838 cites W145594707 @default.
- W2069181838 cites W1579679099 @default.
- W2069181838 cites W1784959505 @default.
- W2069181838 cites W1973746720 @default.
- W2069181838 cites W1975887373 @default.
- W2069181838 cites W1995638133 @default.
- W2069181838 cites W1998141528 @default.
- W2069181838 cites W2001627666 @default.
- W2069181838 cites W2015514617 @default.
- W2069181838 cites W2032288367 @default.
- W2069181838 cites W2035692255 @default.
- W2069181838 cites W2041212096 @default.
- W2069181838 cites W2050455849 @default.
- W2069181838 cites W2065152600 @default.
- W2069181838 cites W2067487487 @default.
- W2069181838 cites W2073282197 @default.
- W2069181838 cites W2082662891 @default.
- W2069181838 cites W2085889967 @default.
- W2069181838 cites W3179911523 @default.
- W2069181838 cites W4245425332 @default.
- W2069181838 cites W4247059482 @default.
- W2069181838 doi "https://doi.org/10.1016/0003-2697(89)90404-1" @default.
- W2069181838 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2817372" @default.
- W2069181838 hasPublicationYear "1989" @default.
- W2069181838 type Work @default.
- W2069181838 sameAs 2069181838 @default.
- W2069181838 citedByCount "6" @default.
- W2069181838 crossrefType "journal-article" @default.
- W2069181838 hasAuthorship W2069181838A5020075142 @default.
- W2069181838 hasAuthorship W2069181838A5044586504 @default.
- W2069181838 hasAuthorship W2069181838A5060128303 @default.
- W2069181838 hasAuthorship W2069181838A5061619473 @default.
- W2069181838 hasAuthorship W2069181838A5085655903 @default.
- W2069181838 hasConcept C111368507 @default.
- W2069181838 hasConcept C127313418 @default.
- W2069181838 hasConcept C181199279 @default.
- W2069181838 hasConcept C185592680 @default.
- W2069181838 hasConcept C2777289219 @default.
- W2069181838 hasConcept C2779920670 @default.
- W2069181838 hasConcept C2780240981 @default.
- W2069181838 hasConcept C2780923616 @default.
- W2069181838 hasConcept C33633552 @default.
- W2069181838 hasConcept C43617362 @default.
- W2069181838 hasConcept C501593827 @default.
- W2069181838 hasConcept C523546767 @default.
- W2069181838 hasConcept C54355233 @default.
- W2069181838 hasConcept C553450214 @default.
- W2069181838 hasConcept C55493867 @default.
- W2069181838 hasConcept C86803240 @default.
- W2069181838 hasConceptScore W2069181838C111368507 @default.
- W2069181838 hasConceptScore W2069181838C127313418 @default.
- W2069181838 hasConceptScore W2069181838C181199279 @default.
- W2069181838 hasConceptScore W2069181838C185592680 @default.
- W2069181838 hasConceptScore W2069181838C2777289219 @default.
- W2069181838 hasConceptScore W2069181838C2779920670 @default.
- W2069181838 hasConceptScore W2069181838C2780240981 @default.
- W2069181838 hasConceptScore W2069181838C2780923616 @default.
- W2069181838 hasConceptScore W2069181838C33633552 @default.
- W2069181838 hasConceptScore W2069181838C43617362 @default.
- W2069181838 hasConceptScore W2069181838C501593827 @default.
- W2069181838 hasConceptScore W2069181838C523546767 @default.
- W2069181838 hasConceptScore W2069181838C54355233 @default.
- W2069181838 hasConceptScore W2069181838C553450214 @default.
- W2069181838 hasConceptScore W2069181838C55493867 @default.
- W2069181838 hasConceptScore W2069181838C86803240 @default.
- W2069181838 hasIssue "1" @default.
- W2069181838 hasLocation W20691818381 @default.
- W2069181838 hasLocation W20691818382 @default.
- W2069181838 hasOpenAccess W2069181838 @default.
- W2069181838 hasPrimaryLocation W20691818381 @default.
- W2069181838 hasRelatedWork W1971041110 @default.
- W2069181838 hasRelatedWork W1976002480 @default.
- W2069181838 hasRelatedWork W1983912328 @default.
- W2069181838 hasRelatedWork W1996284845 @default.
- W2069181838 hasRelatedWork W2000655475 @default.
- W2069181838 hasRelatedWork W2171525001 @default.
- W2069181838 hasRelatedWork W2406666506 @default.
- W2069181838 hasRelatedWork W2415196840 @default.
- W2069181838 hasRelatedWork W2443727728 @default.
- W2069181838 hasRelatedWork W3005406386 @default.
- W2069181838 hasVolume "181" @default.
- W2069181838 isParatext "false" @default.
- W2069181838 isRetracted "false" @default.
- W2069181838 magId "2069181838" @default.
- W2069181838 workType "article" @default.