FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069195440> ?p ?o ?g. }

• W2069195440 endingPage "2739" @default.
• W2069195440 startingPage "2731" @default.
• W2069195440 abstract "The intrinsic stimulatory potential or potency of a eukaryotic gene activator is controlled by the interaction between the activation domain and the transcriptional machinery. To further understand this interaction, we undertook a biochemical study to identify parameters that could be used to modulate activator potency. We considered how varying the number of activation domains, their flexibility, and the number of promoter sites affects potency in a yeast nuclear extract. The effects of GAL4 derivatives bearing either one, two, or four herpes simplex virus VP16 activation domains (amino acids 413 to 454) were measured on DNA templates containing one or two GAL4 sites in a Saccharomyces cerevisiae nuclear extract. We found that multimerized VP16 activation domains acted synergistically to increase the potency of the activators. The spacing between the activation domains was critical, such that the increased flexibility imparted by a protein linker contributed to increased activator potency. With highly potent activators, the levels of transcription stimulated on a single site were saturating, whereas the stimulatory effect of weaker activators increased with the number of sites. We discuss how these biochemical studies relate to the mechanism of gene activation and synergy in a yeast in vitro system." @default.
• W2069195440 created "2016-06-24" @default.
• W2069195440 creator A5014334747 @default.
• W2069195440 creator A5040925674 @default.
• W2069195440 creator A5044652716 @default.
• W2069195440 creator A5073502369 @default.
• W2069195440 creator A5087451751 @default.
• W2069195440 date "1994-04-01" @default.
• W2069195440 modified "2023-09-27" @default.
• W2069195440 title "Modulating the potency of an activator in a yeast in vitro transcription system" @default.
• W2069195440 cites W1924232164 @default.
• W2069195440 cites W1958059146 @default.
• W2069195440 cites W1970393982 @default.
• W2069195440 cites W1970444492 @default.
• W2069195440 cites W1971960644 @default.
• W2069195440 cites W1977347668 @default.
• W2069195440 cites W1979647722 @default.
• W2069195440 cites W1982669711 @default.
• W2069195440 cites W1982756681 @default.
• W2069195440 cites W1984751884 @default.
• W2069195440 cites W1987189376 @default.
• W2069195440 cites W1998108444 @default.
• W2069195440 cites W2002866511 @default.
• W2069195440 cites W2005914718 @default.
• W2069195440 cites W2015617558 @default.
• W2069195440 cites W2021893003 @default.
• W2069195440 cites W2029836146 @default.
• W2069195440 cites W2032849217 @default.
• W2069195440 cites W2034279963 @default.
• W2069195440 cites W2037489737 @default.
• W2069195440 cites W2040728185 @default.
• W2069195440 cites W2041309113 @default.
• W2069195440 cites W2045848136 @default.
• W2069195440 cites W2054435912 @default.
• W2069195440 cites W2056933632 @default.
• W2069195440 cites W2060484288 @default.
• W2069195440 cites W2065679706 @default.
• W2069195440 cites W2068577315 @default.
• W2069195440 cites W2070397307 @default.
• W2069195440 cites W2083951909 @default.
• W2069195440 cites W2088163601 @default.
• W2069195440 cites W2090554343 @default.
• W2069195440 cites W2091160336 @default.
• W2069195440 cites W2091423718 @default.
• W2069195440 cites W2103957060 @default.
• W2069195440 cites W2105014300 @default.
• W2069195440 cites W2117272440 @default.
• W2069195440 cites W2130258789 @default.
• W2069195440 cites W2147634528 @default.
• W2069195440 cites W2164099611 @default.
• W2069195440 cites W2164437319 @default.
• W2069195440 cites W2168978412 @default.
• W2069195440 cites W2204067386 @default.
• W2069195440 cites W4246505086 @default.
• W2069195440 doi "https://doi.org/10.1128/mcb.14.4.2731-2739.1994" @default.
• W2069195440 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/358639" @default.
• W2069195440 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8139572" @default.
• W2069195440 hasPublicationYear "1994" @default.
• W2069195440 type Work @default.
• W2069195440 sameAs 2069195440 @default.
• W2069195440 citedByCount "15" @default.
• W2069195440 crossrefType "journal-article" @default.
• W2069195440 hasAuthorship W2069195440A5014334747 @default.
• W2069195440 hasAuthorship W2069195440A5040925674 @default.
• W2069195440 hasAuthorship W2069195440A5044652716 @default.
• W2069195440 hasAuthorship W2069195440A5073502369 @default.
• W2069195440 hasAuthorship W2069195440A5087451751 @default.
• W2069195440 hasConcept C104317684 @default.
• W2069195440 hasConcept C138885662 @default.
• W2069195440 hasConcept C153911025 @default.
• W2069195440 hasConcept C179926584 @default.
• W2069195440 hasConcept C202751555 @default.
• W2069195440 hasConcept C2777576037 @default.
• W2069195440 hasConcept C2779222958 @default.
• W2069195440 hasConcept C41895202 @default.
• W2069195440 hasConcept C55493867 @default.
• W2069195440 hasConcept C57992300 @default.
• W2069195440 hasConcept C86339819 @default.
• W2069195440 hasConcept C86803240 @default.
• W2069195440 hasConcept C88045685 @default.
• W2069195440 hasConcept C95444343 @default.
• W2069195440 hasConceptScore W2069195440C104317684 @default.
• W2069195440 hasConceptScore W2069195440C138885662 @default.
• W2069195440 hasConceptScore W2069195440C153911025 @default.
• W2069195440 hasConceptScore W2069195440C179926584 @default.
• W2069195440 hasConceptScore W2069195440C202751555 @default.
• W2069195440 hasConceptScore W2069195440C2777576037 @default.
• W2069195440 hasConceptScore W2069195440C2779222958 @default.
• W2069195440 hasConceptScore W2069195440C41895202 @default.
• W2069195440 hasConceptScore W2069195440C55493867 @default.
• W2069195440 hasConceptScore W2069195440C57992300 @default.
• W2069195440 hasConceptScore W2069195440C86339819 @default.
• W2069195440 hasConceptScore W2069195440C86803240 @default.
• W2069195440 hasConceptScore W2069195440C88045685 @default.
• W2069195440 hasConceptScore W2069195440C95444343 @default.
• W2069195440 hasIssue "4" @default.
• W2069195440 hasLocation W20691954401 @default.
• W2069195440 hasOpenAccess W2069195440 @default.

• next