Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069197713> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2069197713 endingPage "245" @default.
- W2069197713 startingPage "242" @default.
- W2069197713 abstract "2-Chloroprocaine is rapidly metabolized in the blood to yield 2-chloro-para-aminobenzoic acid (an inactive metabolite) and diethylaminoethanol (DEAE). DEAE possesses local anesthetic activity. The only reported assay for DEAE is a colorimetric method.Clinical samples of whole blood and serum were obtained from patients receiving stepped intravenous infusions of 3% 2-chloroprocaine. A high pH-dependent liquid-liquid extraction step with diethyl ether was used to eliminate interfering peaks in high-pressure liquid chromatography (HPLC) analysis. Separation and quantitation were performed using HPLC on a polymeric-reversed phase column with a mobile phase consisting of 10% or 20% acetonitrile (for whole blood or serum analysis, respectively) in 50 mm aqueous sodium phosphate buffer, pH = 11.50. The elution order of DEAE and its analogues was tested to interpret the HPLC separation mechanism.Extraction recovery of DEAE from whole blood was 67 +/- 13.5%, from serum, 71 +/- 12.2%, and from water, 75 +/- 2.9%. The high pH value of the mobile phase resulted in sharp, well-resolved peaks with run times of approximately 8 minutes using 20% acetonitrile. The lower limit of detection was 5 ng/mL of DEAE from a 1-mL sample. The elution order of DEAE and its analogues indicated that separation was based on the hydrophobicity of the analytes rather than polar group interactions occurring with silica-based stationary phase.A new, simple and rapid HPLC method for extraction and measurement of DEAE in whole blood or serum samples is reported here." @default.
- W2069197713 created "2016-06-24" @default.
- W2069197713 creator A5010341888 @default.
- W2069197713 creator A5048254369 @default.
- W2069197713 creator A5054217000 @default.
- W2069197713 creator A5071480881 @default.
- W2069197713 creator A5074819448 @default.
- W2069197713 date "1999-05-01" @default.
- W2069197713 modified "2023-09-25" @default.
- W2069197713 title "High-performance liquid chromatographic analysis of the 2-chloroprocaine metabolite, diethylaminoethanol, in blood and serum" @default.
- W2069197713 cites W1969941604 @default.
- W2069197713 cites W1971340456 @default.
- W2069197713 cites W1979646248 @default.
- W2069197713 cites W1996880319 @default.
- W2069197713 cites W1998277621 @default.
- W2069197713 cites W2020224172 @default.
- W2069197713 cites W2021855970 @default.
- W2069197713 cites W2028504558 @default.
- W2069197713 cites W2052924884 @default.
- W2069197713 cites W2054475935 @default.
- W2069197713 cites W2076696791 @default.
- W2069197713 cites W2076837630 @default.
- W2069197713 cites W2113335681 @default.
- W2069197713 cites W2113362276 @default.
- W2069197713 cites W2119586905 @default.
- W2069197713 cites W2253721910 @default.
- W2069197713 cites W2415956023 @default.
- W2069197713 cites W2428139764 @default.
- W2069197713 cites W2947585470 @default.
- W2069197713 doi "https://doi.org/10.1016/s1098-7339(99)90135-9" @default.
- W2069197713 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10338175" @default.
- W2069197713 hasPublicationYear "1999" @default.
- W2069197713 type Work @default.
- W2069197713 sameAs 2069197713 @default.
- W2069197713 citedByCount "0" @default.
- W2069197713 crossrefType "journal-article" @default.
- W2069197713 hasAuthorship W2069197713A5010341888 @default.
- W2069197713 hasAuthorship W2069197713A5048254369 @default.
- W2069197713 hasAuthorship W2069197713A5054217000 @default.
- W2069197713 hasAuthorship W2069197713A5071480881 @default.
- W2069197713 hasAuthorship W2069197713A5074819448 @default.
- W2069197713 hasConcept C10390740 @default.
- W2069197713 hasConcept C141071460 @default.
- W2069197713 hasConcept C1621761 @default.
- W2069197713 hasConcept C179998833 @default.
- W2069197713 hasConcept C185592680 @default.
- W2069197713 hasConcept C2776721371 @default.
- W2069197713 hasConcept C2777463227 @default.
- W2069197713 hasConcept C2777477808 @default.
- W2069197713 hasConcept C43617362 @default.
- W2069197713 hasConcept C4725764 @default.
- W2069197713 hasConcept C55493867 @default.
- W2069197713 hasConcept C71924100 @default.
- W2069197713 hasConcept C73826308 @default.
- W2069197713 hasConcept C95986675 @default.
- W2069197713 hasConceptScore W2069197713C10390740 @default.
- W2069197713 hasConceptScore W2069197713C141071460 @default.
- W2069197713 hasConceptScore W2069197713C1621761 @default.
- W2069197713 hasConceptScore W2069197713C179998833 @default.
- W2069197713 hasConceptScore W2069197713C185592680 @default.
- W2069197713 hasConceptScore W2069197713C2776721371 @default.
- W2069197713 hasConceptScore W2069197713C2777463227 @default.
- W2069197713 hasConceptScore W2069197713C2777477808 @default.
- W2069197713 hasConceptScore W2069197713C43617362 @default.
- W2069197713 hasConceptScore W2069197713C4725764 @default.
- W2069197713 hasConceptScore W2069197713C55493867 @default.
- W2069197713 hasConceptScore W2069197713C71924100 @default.
- W2069197713 hasConceptScore W2069197713C73826308 @default.
- W2069197713 hasConceptScore W2069197713C95986675 @default.
- W2069197713 hasIssue "3" @default.
- W2069197713 hasLocation W20691977131 @default.
- W2069197713 hasLocation W20691977132 @default.
- W2069197713 hasOpenAccess W2069197713 @default.
- W2069197713 hasPrimaryLocation W20691977131 @default.
- W2069197713 hasRelatedWork W1601382037 @default.
- W2069197713 hasRelatedWork W1973892552 @default.
- W2069197713 hasRelatedWork W1987478764 @default.
- W2069197713 hasRelatedWork W2001894285 @default.
- W2069197713 hasRelatedWork W2002567025 @default.
- W2069197713 hasRelatedWork W2049606890 @default.
- W2069197713 hasRelatedWork W2054851034 @default.
- W2069197713 hasRelatedWork W2056980537 @default.
- W2069197713 hasRelatedWork W2064885226 @default.
- W2069197713 hasRelatedWork W2156377834 @default.
- W2069197713 hasVolume "24" @default.
- W2069197713 isParatext "false" @default.
- W2069197713 isRetracted "false" @default.
- W2069197713 magId "2069197713" @default.
- W2069197713 workType "article" @default.