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- W2069300214 abstract "Snail-gut juice was used to prepare protoplasts from Geotrichum lactis. The highest yield of protoplasts was obtained with 0.8 m mannitol as the osmotic stabilizer and with dithiothreitol in the incubation mixture. The protoplasts were osmotically sensitive and burst when suspended in water. Electron microscopy showed that mature protoplasts lacked a cell wall. When treated with the fluorescent brightener, Calcofluor, protoplasts did not fluoresce but hyphal walls (even of empty hyphal compartments) fluoresced strongly, particularly at the tips of hyphae and where septa were present. Calcofluor, which thus binds to carbohydrates of the cell wall but not, apparently, to the intracellular carbohydrates of intact protoplasts, may therefore be used to define the purity of protoplast suspensions." @default.
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- W2069300214 date "1972-08-01" @default.
- W2069300214 modified "2023-09-27" @default.
- W2069300214 title "Preparation of protoplasts from Geotrichum lactis and use of fluorescence to detect cell walls" @default.
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- W2069300214 doi "https://doi.org/10.1016/s0007-1536(72)80043-3" @default.
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