FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069319993> ?p ?o ?g. }

• W2069319993 endingPage "e46826" @default.
• W2069319993 startingPage "e46826" @default.
• W2069319993 abstract "The extracellular biofilm matrix includes primarily DNA and exopolysaccharides (EPS), which function to maintain aggregate structures and to protect biofilms from antibiotics and the immune response. Both polymers are anionic and have cation binding activity, however the impact of this activity on biofilms is not fully understood. Host cell contact is considered the primary signal for activation of most type III secretion systems (T3SS), although calcium limitation is frequently used as a trigger of contact-independent T3SS expression. We hypothesized that alginate, which is a known calcium binding exopolysaccharide produced in mucoid Pseudomonas aeruginosa isolates, can activate the T3SS in biofilms. The addition of exogenous purified alginate to planktonic, non-mucoid PAO1 cultures induced expression of exoS, exoT and exoY-lux reporters of the T3SS in a concentration-dependent manner. Induction by alginate was comparable to induction by the calcium chelator NTA. We extended our analysis of the T3SS in flow chamber-cultivated biofilms, and showed that hyperproduction of alginate in mucA22 mucoid isolates resulted in induction of the exoS-gfp transcriptional reporter compared to non-mucoid paired isolates. We confirmed the transcriptional effects of alginate on the T3SS expression using a FlAsH fluorescence method and showed high levels of the ExoT-Cys(4) protein in mucoid biofilms. Induction of the T3SS could be prevented in planktonic cultures and mucoid biofilms treated with excess calcium, indicating that Ca(2+) chelation by the EPS matrix caused contact-independent induction. However, mucoid isolates generally had reduced exoS-lux expression in comparison to paired, non-mucoid isolates when grown as planktonic cultures and agar colonies. In summary, we have shown a mucoid biofilm-specific induction of the type III secretion system and highlight a difference between planktonic and biofilm cultures in the production of virulence factors." @default.
• W2069319993 created "2016-06-24" @default.
• W2069319993 creator A5047564751 @default.
• W2069319993 creator A5049385540 @default.
• W2069319993 creator A5049718580 @default.
• W2069319993 date "2012-10-08" @default.
• W2069319993 modified "2023-09-27" @default.
• W2069319993 title "Calcium Chelation by Alginate Activates the Type III Secretion System in Mucoid Pseudomonas aeruginosa Biofilms" @default.
• W2069319993 cites W1509261556 @default.
• W2069319993 cites W1555657499 @default.
• W2069319993 cites W1600715779 @default.
• W2069319993 cites W1829749105 @default.
• W2069319993 cites W1832951039 @default.
• W2069319993 cites W1858025662 @default.
• W2069319993 cites W1968589725 @default.
• W2069319993 cites W1970800237 @default.
• W2069319993 cites W1973562367 @default.
• W2069319993 cites W1974157620 @default.
• W2069319993 cites W1974679977 @default.
• W2069319993 cites W1974993545 @default.
• W2069319993 cites W1976680916 @default.
• W2069319993 cites W1994401592 @default.
• W2069319993 cites W1996442931 @default.
• W2069319993 cites W2004668209 @default.
• W2069319993 cites W2020486543 @default.
• W2069319993 cites W2021863804 @default.
• W2069319993 cites W2050130319 @default.
• W2069319993 cites W2050588950 @default.
• W2069319993 cites W2051975001 @default.
• W2069319993 cites W2056137958 @default.
• W2069319993 cites W2056525859 @default.
• W2069319993 cites W2067906280 @default.
• W2069319993 cites W2089190407 @default.
• W2069319993 cites W2098159838 @default.
• W2069319993 cites W2101695227 @default.
• W2069319993 cites W2102677849 @default.
• W2069319993 cites W2110367525 @default.
• W2069319993 cites W2122115838 @default.
• W2069319993 cites W2126887413 @default.
• W2069319993 cites W2127060683 @default.
• W2069319993 cites W2138772877 @default.
• W2069319993 cites W2139761661 @default.
• W2069319993 cites W2144440398 @default.
• W2069319993 cites W2144468707 @default.
• W2069319993 cites W2148674193 @default.
• W2069319993 cites W2150927592 @default.
• W2069319993 cites W2154923841 @default.
• W2069319993 cites W2156382695 @default.
• W2069319993 cites W2163023456 @default.
• W2069319993 cites W2163803918 @default.
• W2069319993 cites W2164386116 @default.
• W2069319993 cites W2370170892 @default.
• W2069319993 cites W2417986161 @default.
• W2069319993 doi "https://doi.org/10.1371/journal.pone.0046826" @default.
• W2069319993 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3466208" @default.
• W2069319993 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23056471" @default.
• W2069319993 hasPublicationYear "2012" @default.
• W2069319993 type Work @default.
• W2069319993 sameAs 2069319993 @default.
• W2069319993 citedByCount "30" @default.
• W2069319993 countsByYear W20693199932013 @default.
• W2069319993 countsByYear W20693199932014 @default.
• W2069319993 countsByYear W20693199932015 @default.
• W2069319993 countsByYear W20693199932016 @default.
• W2069319993 countsByYear W20693199932017 @default.
• W2069319993 countsByYear W20693199932018 @default.
• W2069319993 countsByYear W20693199932019 @default.
• W2069319993 countsByYear W20693199932020 @default.
• W2069319993 countsByYear W20693199932022 @default.
• W2069319993 countsByYear W20693199932023 @default.
• W2069319993 crossrefType "journal-article" @default.
• W2069319993 hasAuthorship W2069319993A5047564751 @default.
• W2069319993 hasAuthorship W2069319993A5049385540 @default.
• W2069319993 hasAuthorship W2069319993A5049718580 @default.
• W2069319993 hasBestOaLocation W20693199931 @default.
• W2069319993 hasConcept C104317684 @default.
• W2069319993 hasConcept C129968341 @default.
• W2069319993 hasConcept C130211564 @default.
• W2069319993 hasConcept C178790620 @default.
• W2069319993 hasConcept C185592680 @default.
• W2069319993 hasConcept C189165786 @default.
• W2069319993 hasConcept C2777637488 @default.
• W2069319993 hasConcept C2778713300 @default.
• W2069319993 hasConcept C28406088 @default.
• W2069319993 hasConcept C519063684 @default.
• W2069319993 hasConcept C523546767 @default.
• W2069319993 hasConcept C54355233 @default.
• W2069319993 hasConcept C55493867 @default.
• W2069319993 hasConcept C58123911 @default.
• W2069319993 hasConcept C60987743 @default.
• W2069319993 hasConcept C86803240 @default.
• W2069319993 hasConcept C89423630 @default.
• W2069319993 hasConceptScore W2069319993C104317684 @default.
• W2069319993 hasConceptScore W2069319993C129968341 @default.
• W2069319993 hasConceptScore W2069319993C130211564 @default.
• W2069319993 hasConceptScore W2069319993C178790620 @default.
• W2069319993 hasConceptScore W2069319993C185592680 @default.
• W2069319993 hasConceptScore W2069319993C189165786 @default.

• next