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• W2069352607 startingPage "1141" @default.
• W2069352607 abstract "We synthesized the m-maleimidobenzoyl derivative of digoxigenin-3-0-succinate (through a p-phenylenediamine bridge) as a hapten derivative directed towards coupling to sulfhydryl grouos of beta-galactosidase. Prepared enzyme conjugate had about 97% of the enzyme labeled with the hapten derivative while retaining full enzyme activity. The enzyme immunoassay for digoxin we prepared showed a maximum sensitivity of 30 pg per assay (c.v. = 3%) with minimal cross-reaction with digotoxin (3.8%). Our method for hapten conjugation to beta-galactosidase is highly efficient and is simple and easily replicated." @default.
• W2069352607 created "2016-06-24" @default.
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• W2069352607 date "1980-10-01" @default.
• W2069352607 modified "2023-09-28" @default.
• W2069352607 title "Quantification of digoxin by enzyme immunoassay: synthesis of a maleimide derivative of digoxigenin succinate for enzyme coupling" @default.
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• W2069352607 doi "https://doi.org/10.1007/bf01976088" @default.
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