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• W2069353566 abstract "Warfarin can stimulate vascular calcification in vitro via activation of β-catenin signaling and/or inhibition of matrix Gla protein (MGP) carboxylation. Calcification was induced in vascular smooth muscle cells (VSMCs) with therapeutic levels of warfarin in normal calcium and clinically acceptable phosphate levels. Although TGF/BMP and PKA pathways are activated in calcifying VSMCs, pharmacologic analysis reveals that their activation is not contributory. However, β-catenin activity is important because inhibition of β-catenin with shRNA or bioflavonoid quercetin prevents calcification in primary human VSMCs, rodent aortic rings, and rat A10 VSMC line. In the presence of quercetin, reactivation of β-catenin using the glycogen synthase kinase-3β (GSK-3β) inhibitor LiCl restores calcium accumulation, confirming that quercetin mechanism of action hinges on inhibition of the β-catenin pathway. Calcification in VSMCs induced by 10 μm warfarin does not associate with reduced levels of carboxylated MGP, and inhibitory effects of quercetin do not involve induction of MGP carboxylation. Further, down-regulation of MGP by shRNA does not alter the effect of quercetin. These results suggest a new β-catenin-targeting strategy to prevent vascular calcification induced by warfarin and identify quercetin as a potential therapeutic in this pathology.Background: Molecular mechanism(s) of warfarin-induced vascular calcification are not well known.Results: Inhibition of β-catenin signaling with shRNA or quercetin prevents osteoblastic transformation and calcification in VSMCs and calcification in aortic rings treated with warfarin, independent from MGP and protein carboxylation.Conclusion: Quercetin inhibits vascular calcification via β-catenin signaling.Significance: Quercetin may be instrumental in treatment of warfarin-induced vascular calcification. Warfarin can stimulate vascular calcification in vitro via activation of β-catenin signaling and/or inhibition of matrix Gla protein (MGP) carboxylation. Calcification was induced in vascular smooth muscle cells (VSMCs) with therapeutic levels of warfarin in normal calcium and clinically acceptable phosphate levels. Although TGF/BMP and PKA pathways are activated in calcifying VSMCs, pharmacologic analysis reveals that their activation is not contributory. However, β-catenin activity is important because inhibition of β-catenin with shRNA or bioflavonoid quercetin prevents calcification in primary human VSMCs, rodent aortic rings, and rat A10 VSMC line. In the presence of quercetin, reactivation of β-catenin using the glycogen synthase kinase-3β (GSK-3β) inhibitor LiCl restores calcium accumulation, confirming that quercetin mechanism of action hinges on inhibition of the β-catenin pathway. Calcification in VSMCs induced by 10 μm warfarin does not associate with reduced levels of carboxylated MGP, and inhibitory effects of quercetin do not involve induction of MGP carboxylation. Further, down-regulation of MGP by shRNA does not alter the effect of quercetin. These results suggest a new β-catenin-targeting strategy to prevent vascular calcification induced by warfarin and identify quercetin as a potential therapeutic in this pathology. Background: Molecular mechanism(s) of warfarin-induced vascular calcification are not well known. Results: Inhibition of β-catenin signaling with shRNA or quercetin prevents osteoblastic transformation and calcification in VSMCs and calcification in aortic rings treated with warfarin, independent from MGP and protein carboxylation. Conclusion: Quercetin inhibits vascular calcification via β-catenin signaling. Significance: Quercetin may be instrumental in treatment of warfarin-induced vascular calcification." @default.
• W2069353566 created "2016-06-24" @default.
• W2069353566 creator A5034659478 @default.
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• W2069353566 date "2013-01-01" @default.
• W2069353566 modified "2023-09-28" @default.
• W2069353566 title "Quercetin Attenuates Warfarin-induced Vascular Calcification in Vitro Independently from Matrix Gla Protein" @default.
• W2069353566 cites W1506159034 @default.
• W2069353566 cites W1558525590 @default.
• W2069353566 cites W1561751527 @default.
• W2069353566 cites W1891912444 @default.
• W2069353566 cites W1969670678 @default.
• W2069353566 cites W1970882491 @default.
• W2069353566 cites W1973638903 @default.
• W2069353566 cites W1973689502 @default.
• W2069353566 cites W1979374034 @default.
• W2069353566 cites W1983712244 @default.
• W2069353566 cites W1983946999 @default.
• W2069353566 cites W1985945376 @default.
• W2069353566 cites W1995911293 @default.
• W2069353566 cites W2000783941 @default.
• W2069353566 cites W2001994991 @default.
• W2069353566 cites W2008166195 @default.
• W2069353566 cites W2024215200 @default.
• W2069353566 cites W2029509488 @default.
• W2069353566 cites W2030057384 @default.
• W2069353566 cites W2031928445 @default.
• W2069353566 cites W2032896143 @default.
• W2069353566 cites W2040003312 @default.
• W2069353566 cites W2042792668 @default.
• W2069353566 cites W2048423913 @default.
• W2069353566 cites W2048990271 @default.
• W2069353566 cites W2051580972 @default.
• W2069353566 cites W2068677249 @default.
• W2069353566 cites W2070175841 @default.
• W2069353566 cites W2071236821 @default.
• W2069353566 cites W2074918724 @default.
• W2069353566 cites W2080447461 @default.
• W2069353566 cites W2092098140 @default.
• W2069353566 cites W2095206475 @default.
• W2069353566 cites W2101368903 @default.
• W2069353566 cites W2104741338 @default.
• W2069353566 cites W2108116509 @default.
• W2069353566 cites W2111566722 @default.
• W2069353566 cites W2113985697 @default.
• W2069353566 cites W2115629484 @default.
• W2069353566 cites W2121861661 @default.
• W2069353566 cites W2123740493 @default.
• W2069353566 cites W2129737450 @default.
• W2069353566 cites W2130835350 @default.
• W2069353566 cites W2141094065 @default.
• W2069353566 cites W2142826608 @default.
• W2069353566 cites W2144312770 @default.
• W2069353566 cites W2144652526 @default.
• W2069353566 cites W2145878813 @default.
• W2069353566 cites W2148413329 @default.
• W2069353566 cites W2150718942 @default.
• W2069353566 cites W2150762378 @default.
• W2069353566 cites W2155088148 @default.
• W2069353566 cites W2156227990 @default.
• W2069353566 cites W2158972360 @default.
• W2069353566 cites W2159856988 @default.
• W2069353566 cites W2164997406 @default.
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• W2069353566 doi "https://doi.org/10.1074/jbc.m112.368639" @default.
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