Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069405212> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2069405212 endingPage "541" @default.
- W2069405212 startingPage "533" @default.
- W2069405212 abstract "In order to standardize automated scoring for the in vivo micronucleus (MN) test a flow cytometric method which recognized micronucleated polychromatic erythrocytes (MPCE) and micronucleated normochromatic erythrocytes (MNCE) in mouse peripheral blood developed by Grawé et al. (1992) has been modified and applied. Blood samples were purified with 35% percoll solution and stained with the RNA-specific dye thiazole orange (TO) and with the DNA-specific dye Hoechst 33342 (HO) for dual laser flow cytometry. The TO fluorescent signals permitted the discrimination between polychromatic and normochromatic erythrocytes (PCE and NCE). Cytotoxic effects could be assessed by the reduction of PCE counts. The blue fluorescent signals of HO permitted the scoring of MN. The MPCE and MNCE were flow sorted for microscopic analysis and showed that 95% of the sorted cells actually contained MN. Three model chemicals, the clastogen mitomycin C, the aneugen colchicine and the industrial chemical acrylamide were tested at 24 h intervals after single intraperitoneal injection up to 72 h after treatment of male (102/ElxC3H/El)F1 mice. All three chemicals showed a dose-related maximum of the MPCE frequencies at 48 h while the MNCE frequencies stayed within the control range up to 72 h. The data obtained with the flow cytometric method were in good agreement with published results. The flow cytometric technique presented here is a fast, accurate and automated method for quantifying MPCE and MNCE in peripheral blood as an indicator of cytogenetic damage induced in the bone marrow and scored in peripheral blood samples. With minor modifications the technique will also be applicable to bone marrow samples." @default.
- W2069405212 created "2016-06-24" @default.
- W2069405212 creator A5024098850 @default.
- W2069405212 creator A5027059438 @default.
- W2069405212 creator A5030348721 @default.
- W2069405212 creator A5064484679 @default.
- W2069405212 date "1993-01-01" @default.
- W2069405212 modified "2023-10-12" @default.
- W2069405212 title "Flow cytometric detection of micronuclei induced by chemicals in poly- and normochromatic erythrocytes of mouse peripheral blood" @default.
- W2069405212 doi "https://doi.org/10.1093/mutage/8.6.533" @default.
- W2069405212 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8133783" @default.
- W2069405212 hasPublicationYear "1993" @default.
- W2069405212 type Work @default.
- W2069405212 sameAs 2069405212 @default.
- W2069405212 citedByCount "35" @default.
- W2069405212 countsByYear W20694052122012 @default.
- W2069405212 countsByYear W20694052122015 @default.
- W2069405212 countsByYear W20694052122016 @default.
- W2069405212 countsByYear W20694052122018 @default.
- W2069405212 countsByYear W20694052122020 @default.
- W2069405212 countsByYear W20694052122021 @default.
- W2069405212 countsByYear W20694052122022 @default.
- W2069405212 countsByYear W20694052122023 @default.
- W2069405212 crossrefType "journal-article" @default.
- W2069405212 hasAuthorship W2069405212A5024098850 @default.
- W2069405212 hasAuthorship W2069405212A5027059438 @default.
- W2069405212 hasAuthorship W2069405212A5030348721 @default.
- W2069405212 hasAuthorship W2069405212A5064484679 @default.
- W2069405212 hasConcept C141710004 @default.
- W2069405212 hasConcept C153911025 @default.
- W2069405212 hasConcept C178790620 @default.
- W2069405212 hasConcept C185592680 @default.
- W2069405212 hasConcept C202751555 @default.
- W2069405212 hasConcept C203014093 @default.
- W2069405212 hasConcept C207001950 @default.
- W2069405212 hasConcept C2776639595 @default.
- W2069405212 hasConcept C2779338040 @default.
- W2069405212 hasConcept C2780007613 @default.
- W2069405212 hasConcept C29730261 @default.
- W2069405212 hasConcept C36681516 @default.
- W2069405212 hasConcept C45080847 @default.
- W2069405212 hasConcept C48679268 @default.
- W2069405212 hasConcept C54355233 @default.
- W2069405212 hasConcept C552990157 @default.
- W2069405212 hasConcept C553184892 @default.
- W2069405212 hasConcept C55493867 @default.
- W2069405212 hasConcept C86803240 @default.
- W2069405212 hasConceptScore W2069405212C141710004 @default.
- W2069405212 hasConceptScore W2069405212C153911025 @default.
- W2069405212 hasConceptScore W2069405212C178790620 @default.
- W2069405212 hasConceptScore W2069405212C185592680 @default.
- W2069405212 hasConceptScore W2069405212C202751555 @default.
- W2069405212 hasConceptScore W2069405212C203014093 @default.
- W2069405212 hasConceptScore W2069405212C207001950 @default.
- W2069405212 hasConceptScore W2069405212C2776639595 @default.
- W2069405212 hasConceptScore W2069405212C2779338040 @default.
- W2069405212 hasConceptScore W2069405212C2780007613 @default.
- W2069405212 hasConceptScore W2069405212C29730261 @default.
- W2069405212 hasConceptScore W2069405212C36681516 @default.
- W2069405212 hasConceptScore W2069405212C45080847 @default.
- W2069405212 hasConceptScore W2069405212C48679268 @default.
- W2069405212 hasConceptScore W2069405212C54355233 @default.
- W2069405212 hasConceptScore W2069405212C552990157 @default.
- W2069405212 hasConceptScore W2069405212C553184892 @default.
- W2069405212 hasConceptScore W2069405212C55493867 @default.
- W2069405212 hasConceptScore W2069405212C86803240 @default.
- W2069405212 hasIssue "6" @default.
- W2069405212 hasLocation W20694052121 @default.
- W2069405212 hasLocation W20694052122 @default.
- W2069405212 hasOpenAccess W2069405212 @default.
- W2069405212 hasPrimaryLocation W20694052121 @default.
- W2069405212 hasRelatedWork W1964557284 @default.
- W2069405212 hasRelatedWork W1987309517 @default.
- W2069405212 hasRelatedWork W2007253861 @default.
- W2069405212 hasRelatedWork W2030573484 @default.
- W2069405212 hasRelatedWork W2135877090 @default.
- W2069405212 hasRelatedWork W2138555681 @default.
- W2069405212 hasRelatedWork W2186646977 @default.
- W2069405212 hasRelatedWork W2347249814 @default.
- W2069405212 hasRelatedWork W2409372136 @default.
- W2069405212 hasRelatedWork W4246869807 @default.
- W2069405212 hasVolume "8" @default.
- W2069405212 isParatext "false" @default.
- W2069405212 isRetracted "false" @default.
- W2069405212 magId "2069405212" @default.
- W2069405212 workType "article" @default.