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• W2069433022 startingPage "8765" @default.
• W2069433022 abstract "Double electron−electron resonance (DEER), a pulsed electron paramagnetic resonance (EPR) spectroscopy technique, was utilized to characterize conformational population shifts in HIV-1 protease (HIV-1PR) upon interaction with various inhibitors. Distances between spin-labeled sites in the flap region of HIV-1PR were determined, and detailed analyses provide population percentages for the ensemble flap conformations upon interaction with inhibitor or substrate. Comparisons are made between the percentage of the closed conformer seen with DEER and enzymatic inhibition constants, thermodynamic dissociation constants, and the number of hydrogen bonds identified in crystallographic complexes." @default.
• W2069433022 created "2016-06-24" @default.
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• W2069433022 date "2009-08-24" @default.
• W2069433022 modified "2023-10-03" @default.
• W2069433022 title "Monitoring Inhibitor-Induced Conformational Population Shifts in HIV-1 Protease by Pulsed EPR Spectroscopy" @default.
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• W2069433022 doi "https://doi.org/10.1021/bi901201q" @default.
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• W2069433022 hasPublicationYear "2009" @default.
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