FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069448070> ?p ?o ?g. }

• W2069448070 endingPage "686" @default.
• W2069448070 startingPage "675" @default.
• W2069448070 abstract "Long-term immunization of rabbits with postsynaptic densities (PSD) from bovine brain produced an antiserum specific for PSD as judged by binding to subcellular fractions and immunohistochemical location at the light and electron microscope levels. (a) The major antigens of bovine PSD preparations were three polypeptides of molecular weight 95,000 (PSD-95), 82,000 (PSD-82), and 72,000 (PSD-72), respectively. Antigen PSD-95, also present in mouse and rat PSDs was virtually absent from cytoplasm, myelin, mitochondria, and microsomes from rodent or bovine brain. Antigens PSD-82 and PSD-72 were present in all subcellular fractions from bovine brain, especially in mitochondria, but were almost absent from rodent brain. The antiserum also contained low-affinity antibodies against tubulin. (b)Immunohistochemical studies were performed in mouse and rat brain, where antigen PSD-95 accounted for 90 percent of the antiserum binding after adsorption with purified brain tubulin. At the light microscope level, antibody binding was observed only in those regions of the brain where synapses are known to be present. No reaction was observed in myelinated tracts, in the neuronal cytoplasm, or in nonneuronal cells. Strong reactivity was observed in the molecular layer of the dentate gyrus, stratum oriens and stratum radiatum of the hippocampus, and the molecular layer of the cerebellum. Experimental lesions, such as ablation of the rat entorhinal cortex or intraventricular injection of kainic acid, which led to a major loss of PSD in well- defined areas of the hippocampal formation, caused a correlative decrease in immunoreactivity in these areas. Abnormal patterns of immunohistochemical staining correlated with abnormal synaptic patterns in the cerebella of reeler and staggerer mouse mutants. (c) At the electron microscopic level, immunoreactivity was detectable only in PSD. The antibody did not bind to myelin, mitochondria or plasma membranes. (d) The results indicate that antigen PSD-95 is located predominantly or exclusively in PSD and can be used as a marker during subcellular fractionation. Other potential uses include the study of synaptogenesis, and the detection of changes in synapse number after experimental perturbations of the nervous system." @default.
• W2069448070 created "2016-06-24" @default.
• W2069448070 creator A5029331773 @default.
• W2069448070 creator A5029827328 @default.
• W2069448070 creator A5063032668 @default.
• W2069448070 date "1981-09-01" @default.
• W2069448070 modified "2023-10-04" @default.
• W2069448070 title "Postsynaptic density antigens: preparation and characterization of an antiserum against postsynaptic densities" @default.
• W2069448070 cites W1571270189 @default.
• W2069448070 cites W1618384257 @default.
• W2069448070 cites W1775749144 @default.
• W2069448070 cites W1914200757 @default.
• W2069448070 cites W1975051152 @default.
• W2069448070 cites W1977251643 @default.
• W2069448070 cites W1977773125 @default.
• W2069448070 cites W1982180324 @default.
• W2069448070 cites W1990661088 @default.
• W2069448070 cites W1997246394 @default.
• W2069448070 cites W2005143182 @default.
• W2069448070 cites W2006154471 @default.
• W2069448070 cites W200757568 @default.
• W2069448070 cites W2019126938 @default.
• W2069448070 cites W2020357090 @default.
• W2069448070 cites W2028058524 @default.
• W2069448070 cites W2038089434 @default.
• W2069448070 cites W2052664734 @default.
• W2069448070 cites W2057473661 @default.
• W2069448070 cites W2068850000 @default.
• W2069448070 cites W2070900774 @default.
• W2069448070 cites W2075875603 @default.
• W2069448070 cites W2086450006 @default.
• W2069448070 cites W2087587830 @default.
• W2069448070 cites W2093480892 @default.
• W2069448070 cites W2097790982 @default.
• W2069448070 cites W2131364970 @default.
• W2069448070 cites W2137181530 @default.
• W2069448070 cites W2139834955 @default.
• W2069448070 cites W2146155881 @default.
• W2069448070 cites W2148297185 @default.
• W2069448070 cites W2167773427 @default.
• W2069448070 cites W2169787405 @default.
• W2069448070 cites W2220782623 @default.
• W2069448070 doi "https://doi.org/10.1083/jcb.90.3.675" @default.
• W2069448070 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2111895" @default.
• W2069448070 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6169734" @default.
• W2069448070 hasPublicationYear "1981" @default.
• W2069448070 type Work @default.
• W2069448070 sameAs 2069448070 @default.
• W2069448070 citedByCount "34" @default.
• W2069448070 countsByYear W20694480702012 @default.
• W2069448070 countsByYear W20694480702013 @default.
• W2069448070 countsByYear W20694480702014 @default.
• W2069448070 countsByYear W20694480702015 @default.
• W2069448070 countsByYear W20694480702017 @default.
• W2069448070 countsByYear W20694480702020 @default.
• W2069448070 countsByYear W20694480702021 @default.
• W2069448070 countsByYear W20694480702023 @default.
• W2069448070 crossrefType "journal-article" @default.
• W2069448070 hasAuthorship W2069448070A5029331773 @default.
• W2069448070 hasAuthorship W2069448070A5029827328 @default.
• W2069448070 hasAuthorship W2069448070A5063032668 @default.
• W2069448070 hasBestOaLocation W20694480701 @default.
• W2069448070 hasConcept C134018914 @default.
• W2069448070 hasConcept C147483822 @default.
• W2069448070 hasConcept C148762608 @default.
• W2069448070 hasConcept C153911025 @default.
• W2069448070 hasConcept C170493617 @default.
• W2069448070 hasConcept C187541742 @default.
• W2069448070 hasConcept C190062978 @default.
• W2069448070 hasConcept C197341189 @default.
• W2069448070 hasConcept C203014093 @default.
• W2069448070 hasConcept C2776464000 @default.
• W2069448070 hasConcept C2779652256 @default.
• W2069448070 hasConcept C2780715579 @default.
• W2069448070 hasConcept C2781161787 @default.
• W2069448070 hasConcept C55493867 @default.
• W2069448070 hasConcept C86803240 @default.
• W2069448070 hasConcept C91523082 @default.
• W2069448070 hasConcept C95444343 @default.
• W2069448070 hasConceptScore W2069448070C134018914 @default.
• W2069448070 hasConceptScore W2069448070C147483822 @default.
• W2069448070 hasConceptScore W2069448070C148762608 @default.
• W2069448070 hasConceptScore W2069448070C153911025 @default.
• W2069448070 hasConceptScore W2069448070C170493617 @default.
• W2069448070 hasConceptScore W2069448070C187541742 @default.
• W2069448070 hasConceptScore W2069448070C190062978 @default.
• W2069448070 hasConceptScore W2069448070C197341189 @default.
• W2069448070 hasConceptScore W2069448070C203014093 @default.
• W2069448070 hasConceptScore W2069448070C2776464000 @default.
• W2069448070 hasConceptScore W2069448070C2779652256 @default.
• W2069448070 hasConceptScore W2069448070C2780715579 @default.
• W2069448070 hasConceptScore W2069448070C2781161787 @default.
• W2069448070 hasConceptScore W2069448070C55493867 @default.
• W2069448070 hasConceptScore W2069448070C86803240 @default.
• W2069448070 hasConceptScore W2069448070C91523082 @default.
• W2069448070 hasConceptScore W2069448070C95444343 @default.
• W2069448070 hasIssue "3" @default.
• W2069448070 hasLocation W20694480701 @default.

• next