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- W2069455572 abstract "Using morphologic, enzyme-cytochemical, and immunocytochemical methods, the functional diversity of the mononuclear phagocyte system can be studied only to a limited extent. Therefore, enzyme-deficient monocyte/macrophage cell lines have been established as technical prerequisites for the generation of monocyte/macrophage hybrids by applying selective media. After mutation with ethylmethanesulfonate, six clones of U-937 were selected against increasing concentrations of 5'-bromodesoxyuridine; these clones are defective in thymidine kinase (EC 2.7.1.21), as shown by autoradiography and direct measurement of [3H]thymidine uptake. A broad marker panel indicates that the clones could be appropriate for the establishment of human monocyte/macrophage hybrids." @default.
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- W2069455572 date "1985-04-01" @default.
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- W2069455572 title "Establishment of thymidine kinase (EC 2.7.1.21)-deficient mutants of human monocytic cell line U-937" @default.
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- W2069455572 doi "https://doi.org/10.1016/0008-8749(85)90247-3" @default.
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