FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069565619> ?p ?o ?g. }

• W2069565619 endingPage "182" @default.
• W2069565619 startingPage "175" @default.
• W2069565619 abstract "To determine if the observed tirapazamine (TPZ)-cisplatin synergistic cell kill was mediated at the cellular level by impairment of upregulation of key proteins involved in repair of DNA interstrand crosslinks. Cisplatin sensitivity has been shown to be dependent on the expression of the two DNA repair proteins ERCC1 and XPA. ERCC1 expression has also been shown to be upregulated by cisplatin exposure. Therefore, these studies were undertaken to determine if hypoxia-activated TPZ pretreatment inhibited the cells' normal protective response to cisplatin via inhibiting the upregulation of ERCC1 and/or XPA expression.Two different cell lines, one cisplatin sensitive and one cisplatin resistant, were treated with TPZ, cisplatin, both drugs together (which results in additive cytotoxicity), or TPZ followed by cisplatin (which results in synergistic cytotoxicity). All cells were exposed to 1 h of hypoxia to bioactivate the TPZ. Expression of ERCC1 and XPA were evaluated at the mRNA and protein level at 24 or 48 h after drug exposure.In the cisplatin-sensitive non-small-cell lung cancer cell line, ERCC1 expression at the mRNA or protein level was not significantly altered in any of the treatment groups. In the cisplatin-resistant ovarian cancer cell line, ERCC1 expression was upregulated by TPZ, but not by cisplatin alone. The change in protein expression was less pronounced than the change in mRNA level. XPA expression was not significantly changed from baseline in either cell line at the mRNA or protein level.In contrast to reports in the literature, this study did not demonstrate cisplatin inducing its own repair by upregulating the DNA crosslink repair proteins ERCC1 or XPA. Therefore, the TPZ-cisplatin synergism cannot be mediated through hypoxia-activated TPZ inhibiting this cellular protective response. TPZ alone, however, was able to alter repair protein expression, which may play a role in mediating its cytotoxicity." @default.
• W2069565619 created "2016-06-24" @default.
• W2069565619 creator A5029071777 @default.
• W2069565619 creator A5029144863 @default.
• W2069565619 creator A5039706503 @default.
• W2069565619 creator A5069210077 @default.
• W2069565619 date "2001-01-01" @default.
• W2069565619 modified "2023-09-27" @default.
• W2069565619 title "An investigation of the molecular basis for the synergistic interaction of tirapazamine and cisplatin" @default.
• W2069565619 cites W1528896526 @default.
• W2069565619 cites W1775562748 @default.
• W2069565619 cites W1802949207 @default.
• W2069565619 cites W1973262735 @default.
• W2069565619 cites W1977382129 @default.
• W2069565619 cites W1994070583 @default.
• W2069565619 cites W1997518044 @default.
• W2069565619 cites W2009245901 @default.
• W2069565619 cites W2015130934 @default.
• W2069565619 cites W2018588892 @default.
• W2069565619 cites W2022409096 @default.
• W2069565619 cites W2033281614 @default.
• W2069565619 cites W2035659472 @default.
• W2069565619 cites W2094795615 @default.
• W2069565619 cites W2098645377 @default.
• W2069565619 cites W2102455302 @default.
• W2069565619 cites W2120000242 @default.
• W2069565619 cites W2145953677 @default.
• W2069565619 cites W2153965661 @default.
• W2069565619 cites W2271870719 @default.
• W2069565619 doi "https://doi.org/10.1016/s0360-3016(00)01379-1" @default.
• W2069565619 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11163512" @default.
• W2069565619 hasPublicationYear "2001" @default.
• W2069565619 type Work @default.
• W2069565619 sameAs 2069565619 @default.
• W2069565619 citedByCount "15" @default.
• W2069565619 countsByYear W20695656192013 @default.
• W2069565619 countsByYear W20695656192022 @default.
• W2069565619 crossrefType "journal-article" @default.
• W2069565619 hasAuthorship W2069565619A5029071777 @default.
• W2069565619 hasAuthorship W2069565619A5029144863 @default.
• W2069565619 hasAuthorship W2069565619A5039706503 @default.
• W2069565619 hasAuthorship W2069565619A5069210077 @default.
• W2069565619 hasConcept C104317684 @default.
• W2069565619 hasConcept C104451858 @default.
• W2069565619 hasConcept C109316439 @default.
• W2069565619 hasConcept C126322002 @default.
• W2069565619 hasConcept C127561419 @default.
• W2069565619 hasConcept C134935766 @default.
• W2069565619 hasConcept C153911025 @default.
• W2069565619 hasConcept C185592680 @default.
• W2069565619 hasConcept C202751555 @default.
• W2069565619 hasConcept C2776313096 @default.
• W2069565619 hasConcept C2776694085 @default.
• W2069565619 hasConcept C2778239845 @default.
• W2069565619 hasConcept C2779309665 @default.
• W2069565619 hasConcept C502942594 @default.
• W2069565619 hasConcept C54355233 @default.
• W2069565619 hasConcept C552990157 @default.
• W2069565619 hasConcept C55493867 @default.
• W2069565619 hasConcept C71924100 @default.
• W2069565619 hasConcept C81885089 @default.
• W2069565619 hasConcept C86803240 @default.
• W2069565619 hasConceptScore W2069565619C104317684 @default.
• W2069565619 hasConceptScore W2069565619C104451858 @default.
• W2069565619 hasConceptScore W2069565619C109316439 @default.
• W2069565619 hasConceptScore W2069565619C126322002 @default.
• W2069565619 hasConceptScore W2069565619C127561419 @default.
• W2069565619 hasConceptScore W2069565619C134935766 @default.
• W2069565619 hasConceptScore W2069565619C153911025 @default.
• W2069565619 hasConceptScore W2069565619C185592680 @default.
• W2069565619 hasConceptScore W2069565619C202751555 @default.
• W2069565619 hasConceptScore W2069565619C2776313096 @default.
• W2069565619 hasConceptScore W2069565619C2776694085 @default.
• W2069565619 hasConceptScore W2069565619C2778239845 @default.
• W2069565619 hasConceptScore W2069565619C2779309665 @default.
• W2069565619 hasConceptScore W2069565619C502942594 @default.
• W2069565619 hasConceptScore W2069565619C54355233 @default.
• W2069565619 hasConceptScore W2069565619C552990157 @default.
• W2069565619 hasConceptScore W2069565619C55493867 @default.
• W2069565619 hasConceptScore W2069565619C71924100 @default.
• W2069565619 hasConceptScore W2069565619C81885089 @default.
• W2069565619 hasConceptScore W2069565619C86803240 @default.
• W2069565619 hasIssue "1" @default.
• W2069565619 hasLocation W20695656191 @default.
• W2069565619 hasLocation W20695656192 @default.
• W2069565619 hasOpenAccess W2069565619 @default.
• W2069565619 hasPrimaryLocation W20695656191 @default.
• W2069565619 hasRelatedWork W1963528972 @default.
• W2069565619 hasRelatedWork W2028622397 @default.
• W2069565619 hasRelatedWork W2099082193 @default.
• W2069565619 hasRelatedWork W2113467423 @default.
• W2069565619 hasRelatedWork W2154925720 @default.
• W2069565619 hasRelatedWork W2155484722 @default.
• W2069565619 hasRelatedWork W2374321762 @default.
• W2069565619 hasRelatedWork W3028670831 @default.
• W2069565619 hasRelatedWork W3096286885 @default.
• W2069565619 hasRelatedWork W57764544 @default.
• W2069565619 hasVolume "49" @default.

• next