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- W2069621993 abstract "changes in human sperm head using cytochemical stains and to confirm that CAP/AR do not include DNA damage. METHODS: Percoll-washed spermatozoa were incubated in BWW / fetal cord serum ultrafiltrate (10%;3.5h;CAP) and, subsequently, with lysophosphatidylcholine (2.5 M; 30 min, AR). Then, ethanol-fixed spermatozoa were used for: PSA-FITC (for AR), aniline blue (AB, for histones), chromomycin A3 (CMA3, for protamines), toluidine blue (TB, for chromatin compaction), iodoacetamide-fluorescein (IAF, for sulfhydryl groups), induced decondensation, sperm chromatin structure assay (DNA fragmentation index, DFI) and immunoblotting (for histone, H2B). RESULTS: CAP/AR was associated with similar increases in staining for AB ( 75%) and TB ( 50%) but had no inflence on CMA3. The increase ( 55%) in IAF staining observed during CAP was abscent after AR. CAP/AR did not damage DNA (DFI remained low) nor affect histone content. CAP, and even more AR, primed sperm heads to decondense ( 100% and 160% increases, respectively) when challenged with SDS (1%) DTT (0.1 mM). Interestingly, induced decondensation correlated with all other tests CAP, AB, TB, IAF; also CAP was related to AB and TB stains. CONCLUSIONS: The data strongly support human sperm chromatin remodeling during CAP/AR, these modifications are interlinked and probably help prepare chromatin for after-fertilization events." @default.
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- W2069621993 date "2012-04-01" @default.
- W2069621993 modified "2023-09-24" @default.
- W2069621993 title "2099 IMPAIRED CLEARANCE OF APOPTOTIC GERM CELLS CAUSES IMPAIRED SPERMATOGENESIS ASSOCIATED WITH LEYDIG CELL HYPERPLASIA AND INCREASED TESTOSTERONE LEVELS" @default.
- W2069621993 doi "https://doi.org/10.1016/j.juro.2012.02.2266" @default.
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