FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069695754> ?p ?o ?g. }

• W2069695754 endingPage "1328" @default.
• W2069695754 startingPage "1319" @default.
• W2069695754 abstract "EP and GPEET procyclin, the major surface glycoproteins of procyclic forms of Trypanosoma brucei, are truncated by proteases in the midgut of the tsetse fly Glossina morsitans morsitans. We show that soluble extracts from the midguts of teneral flies contain trypsin-like enzymes that cleave the N-terminal domains from living culture-derived parasites. The same extract shows little activity against a variant surface glycoprotein on living bloodstream form T. brucei (MITat 1.2) and none against glutamic acid/alanine-rich protein, a major surface glycoprotein of Trypanosoma congolense insect forms although both these proteins contain potential trypsin cleavage sites. Gel filtration of tsetse midgut extract revealed three peaks of tryptic activity against procyclins. Trypsin alone would be sufficient to account for the cleavage of GPEET at a single arginine residue in the fly. In contrast, the processing of EP at multiple sites would require additional enzymes that might only be induced or activated during feeding or infection. Unexpectedly, the pH optima for both the procyclin cleavage reaction and digestion of the trypsin-specific synthetic substrate Chromozym-TRY were extremely alkaline (pH 10). Direct measurements were made of the pH within different compartments of the tsetse digestive tract. We conclude that the gut pH of teneral flies, from the proventriculus to the hindgut, is alkaline, in contradiction to previous measurements indicating that it was mildly acidic. When tsetse flies were analysed 48 h after their first bloodmeal, a pH gradient from the proventriculus (pH 10.6+/-0.6) to the posterior midgut (pH 7.9+/-0.4) was observed." @default.
• W2069695754 created "2016-06-24" @default.
• W2069695754 creator A5046873317 @default.
• W2069695754 creator A5050874457 @default.
• W2069695754 creator A5072571167 @default.
• W2069695754 creator A5073741613 @default.
• W2069695754 creator A5074927575 @default.
• W2069695754 creator A5087425710 @default.
• W2069695754 creator A5090967339 @default.
• W2069695754 date "2003-10-01" @default.
• W2069695754 modified "2023-10-16" @default.
• W2069695754 title "Cleavage of trypanosome surface glycoproteins by alkaline trypsin-like enzyme(s) in the midgut of Glossina morsitans" @default.
• W2069695754 cites W1527626356 @default.
• W2069695754 cites W1578986895 @default.
• W2069695754 cites W1963794675 @default.
• W2069695754 cites W1967039397 @default.
• W2069695754 cites W1967775530 @default.
• W2069695754 cites W1974223831 @default.
• W2069695754 cites W1974939251 @default.
• W2069695754 cites W1985476912 @default.
• W2069695754 cites W1988014151 @default.
• W2069695754 cites W1998057683 @default.
• W2069695754 cites W2013217378 @default.
• W2069695754 cites W2020308781 @default.
• W2069695754 cites W2036648344 @default.
• W2069695754 cites W2038472053 @default.
• W2069695754 cites W2040498921 @default.
• W2069695754 cites W2050083559 @default.
• W2069695754 cites W2057243403 @default.
• W2069695754 cites W2058498493 @default.
• W2069695754 cites W2073689323 @default.
• W2069695754 cites W2080892910 @default.
• W2069695754 cites W2081183303 @default.
• W2069695754 cites W2082919092 @default.
• W2069695754 cites W2084257231 @default.
• W2069695754 cites W2093327788 @default.
• W2069695754 cites W2095177345 @default.
• W2069695754 cites W2108555971 @default.
• W2069695754 cites W2126540162 @default.
• W2069695754 cites W2140548987 @default.
• W2069695754 cites W2157715696 @default.
• W2069695754 cites W2161761672 @default.
• W2069695754 cites W2162487244 @default.
• W2069695754 cites W2189100235 @default.
• W2069695754 cites W2222407951 @default.
• W2069695754 cites W4240445678 @default.
• W2069695754 doi "https://doi.org/10.1016/s0020-7519(03)00182-6" @default.
• W2069695754 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14527515" @default.
• W2069695754 hasPublicationYear "2003" @default.
• W2069695754 type Work @default.
• W2069695754 sameAs 2069695754 @default.
• W2069695754 citedByCount "34" @default.
• W2069695754 countsByYear W20696957542013 @default.
• W2069695754 countsByYear W20696957542014 @default.
• W2069695754 countsByYear W20696957542016 @default.
• W2069695754 countsByYear W20696957542017 @default.
• W2069695754 countsByYear W20696957542018 @default.
• W2069695754 countsByYear W20696957542019 @default.
• W2069695754 countsByYear W20696957542020 @default.
• W2069695754 countsByYear W20696957542021 @default.
• W2069695754 countsByYear W20696957542022 @default.
• W2069695754 countsByYear W20696957542023 @default.
• W2069695754 crossrefType "journal-article" @default.
• W2069695754 hasAuthorship W2069695754A5046873317 @default.
• W2069695754 hasAuthorship W2069695754A5050874457 @default.
• W2069695754 hasAuthorship W2069695754A5072571167 @default.
• W2069695754 hasAuthorship W2069695754A5073741613 @default.
• W2069695754 hasAuthorship W2069695754A5074927575 @default.
• W2069695754 hasAuthorship W2069695754A5087425710 @default.
• W2069695754 hasAuthorship W2069695754A5090967339 @default.
• W2069695754 hasConcept C104317684 @default.
• W2069695754 hasConcept C108625454 @default.
• W2069695754 hasConcept C150149183 @default.
• W2069695754 hasConcept C173758957 @default.
• W2069695754 hasConcept C181199279 @default.
• W2069695754 hasConcept C182220744 @default.
• W2069695754 hasConcept C2779502636 @default.
• W2069695754 hasConcept C2780340462 @default.
• W2069695754 hasConcept C2911108730 @default.
• W2069695754 hasConcept C55493867 @default.
• W2069695754 hasConcept C59822182 @default.
• W2069695754 hasConcept C86803240 @default.
• W2069695754 hasConcept C90856448 @default.
• W2069695754 hasConceptScore W2069695754C104317684 @default.
• W2069695754 hasConceptScore W2069695754C108625454 @default.
• W2069695754 hasConceptScore W2069695754C150149183 @default.
• W2069695754 hasConceptScore W2069695754C173758957 @default.
• W2069695754 hasConceptScore W2069695754C181199279 @default.
• W2069695754 hasConceptScore W2069695754C182220744 @default.
• W2069695754 hasConceptScore W2069695754C2779502636 @default.
• W2069695754 hasConceptScore W2069695754C2780340462 @default.
• W2069695754 hasConceptScore W2069695754C2911108730 @default.
• W2069695754 hasConceptScore W2069695754C55493867 @default.
• W2069695754 hasConceptScore W2069695754C59822182 @default.
• W2069695754 hasConceptScore W2069695754C86803240 @default.
• W2069695754 hasConceptScore W2069695754C90856448 @default.
• W2069695754 hasIssue "12" @default.
• W2069695754 hasLocation W20696957541 @default.

• next