FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069732020> ?p ?o ?g. }

• W2069732020 abstract "The post-meiotic phase of spermatogenesis is characterised by a radical reorganization of the chromatin, leading to its nucleosomal to toroid transition. The replacement of histones with protamine is a gradual process regulated to the hierarchical translation of repressed mRNAs leading to the following events: incorporation of testis-specific histone variants and general histone hyperacetylation, bromodomain proteins, transition proteins, concluded by protamine incorporation on DNA. In this work, we tested whether the induced expression of human protamine 1 (PR1) in sheep somatic cells could induce a protamine/toroid conformation of interphase nuclei. Sheep adult fibroblasts (SAF) were cultured in DMEM with 10% fetal bovine serum from second to eighth passage. Then, SAF at 80% confluence were transfected with 3 μg of pPR1-red fluorescent protein (RFP) and pRFP (CTR) with lipofectamine. At 4 h post-transfection, cells were treated with 5 nM trichostatin A (TSA; histone deacethylase inhibitor) for an additional 16 h. Transfected cells (visualised through the RFP tag) were analysed for nuclear morphology (transmission electron microscopy), PR1-RFP expression (confocal microscope, RT-PCR, Western blot), cytofluorimeter, DNA damage (comet assay, pH2A.X immune-detection), and chromatin immune-precipitation assays (ChIP). Moreover, to visualise the histone/protamine exchange, we transfected with PR1 mouse GFP-H2B fibroblasts. Protaminized cells were used as donors for nuclear transfer (NT) and TH2B (testis/oocyte-specific histone normally present in male pronucleus, a marker of reprogramming) was detected in pronuclear stage of NT zygotes. The χ2 test was used for statistical analyses. We demonstrated that PR1 translocates into the nuclei and gradually compacts them into elongating spermatid-like structures in 48 h; TSA treatment facilitates the process [TSA 83.3% (50/60); without TSA 55.2%; 58/105; P < 0.0003]. A complete histone-to-protamine exchange was also visualised in GFP-H2B nuclei (mouse fibroblasts) 40h after PR1 transfection. Cytofluorimetric analysis demonstrated that protamine incorporation occurs in any cell cycle stage and without DNA breaks. Next, protamine-protamine binding was excluded by ChIP analysis, which confirmed protamine-DNA binding. Finally, protaminized nuclei transplanted into enucleated oocytes incorporated maternal histone TH2B (3/8), whereas no signal was detected in control cells (0/9), suggesting that protaminized nuclei are better remodelled. We conclude that the induced expression of PR1 forces the somatic chromatin to acquire a structure overlapping elongated spermatid/spermatozoa, a conformation that perfectly matches the nuclear reprogramming machinery of the oocyte. Further work will determine whether protaminized cells are better reprogrammed upon nuclear transfer." @default.
• W2069732020 created "2016-06-24" @default.
• W2069732020 creator A5002697302 @default.
• W2069732020 creator A5007199304 @default.
• W2069732020 creator A5024828484 @default.
• W2069732020 creator A5033322034 @default.
• W2069732020 creator A5038731471 @default.
• W2069732020 creator A5045285332 @default.
• W2069732020 creator A5062924194 @default.
• W2069732020 creator A5064681496 @default.
• W2069732020 creator A5071537135 @default.
• W2069732020 creator A5084126816 @default.
• W2069732020 creator A5087867462 @default.
• W2069732020 date "2015-01-01" @default.
• W2069732020 modified "2023-10-02" @default.
• W2069732020 title "42 CONVERSION OF THE CHROMATIN OF SOMATIC CELLS INTO SPERMATID-LIKE STRUCTURES" @default.
• W2069732020 doi "https://doi.org/10.1071/rdv27n1ab42" @default.
• W2069732020 hasPublicationYear "2015" @default.
• W2069732020 type Work @default.
• W2069732020 sameAs 2069732020 @default.
• W2069732020 citedByCount "0" @default.
• W2069732020 crossrefType "journal-article" @default.
• W2069732020 hasAuthorship W2069732020A5002697302 @default.
• W2069732020 hasAuthorship W2069732020A5007199304 @default.
• W2069732020 hasAuthorship W2069732020A5024828484 @default.
• W2069732020 hasAuthorship W2069732020A5033322034 @default.
• W2069732020 hasAuthorship W2069732020A5038731471 @default.
• W2069732020 hasAuthorship W2069732020A5045285332 @default.
• W2069732020 hasAuthorship W2069732020A5062924194 @default.
• W2069732020 hasAuthorship W2069732020A5064681496 @default.
• W2069732020 hasAuthorship W2069732020A5071537135 @default.
• W2069732020 hasAuthorship W2069732020A5084126816 @default.
• W2069732020 hasAuthorship W2069732020A5087867462 @default.
• W2069732020 hasConcept C153911025 @default.
• W2069732020 hasConcept C2777394357 @default.
• W2069732020 hasConcept C2777557582 @default.
• W2069732020 hasConcept C2779085176 @default.
• W2069732020 hasConcept C2780835560 @default.
• W2069732020 hasConcept C2781087480 @default.
• W2069732020 hasConcept C54009773 @default.
• W2069732020 hasConcept C54355233 @default.
• W2069732020 hasConcept C552990157 @default.
• W2069732020 hasConcept C55493867 @default.
• W2069732020 hasConcept C64927066 @default.
• W2069732020 hasConcept C81885089 @default.
• W2069732020 hasConcept C83640560 @default.
• W2069732020 hasConcept C86803240 @default.
• W2069732020 hasConcept C95444343 @default.
• W2069732020 hasConceptScore W2069732020C153911025 @default.
• W2069732020 hasConceptScore W2069732020C2777394357 @default.
• W2069732020 hasConceptScore W2069732020C2777557582 @default.
• W2069732020 hasConceptScore W2069732020C2779085176 @default.
• W2069732020 hasConceptScore W2069732020C2780835560 @default.
• W2069732020 hasConceptScore W2069732020C2781087480 @default.
• W2069732020 hasConceptScore W2069732020C54009773 @default.
• W2069732020 hasConceptScore W2069732020C54355233 @default.
• W2069732020 hasConceptScore W2069732020C552990157 @default.
• W2069732020 hasConceptScore W2069732020C55493867 @default.
• W2069732020 hasConceptScore W2069732020C64927066 @default.
• W2069732020 hasConceptScore W2069732020C81885089 @default.
• W2069732020 hasConceptScore W2069732020C83640560 @default.
• W2069732020 hasConceptScore W2069732020C86803240 @default.
• W2069732020 hasConceptScore W2069732020C95444343 @default.
• W2069732020 hasLocation W20697320201 @default.
• W2069732020 hasOpenAccess W2069732020 @default.
• W2069732020 hasPrimaryLocation W20697320201 @default.
• W2069732020 hasRelatedWork W1041149315 @default.
• W2069732020 hasRelatedWork W1483955803 @default.
• W2069732020 hasRelatedWork W1507059425 @default.
• W2069732020 hasRelatedWork W1561045112 @default.
• W2069732020 hasRelatedWork W1964391265 @default.
• W2069732020 hasRelatedWork W1969899979 @default.
• W2069732020 hasRelatedWork W1972410182 @default.
• W2069732020 hasRelatedWork W1975477949 @default.
• W2069732020 hasRelatedWork W2018720005 @default.
• W2069732020 hasRelatedWork W2062629632 @default.
• W2069732020 hasRelatedWork W2063085696 @default.
• W2069732020 hasRelatedWork W2066980408 @default.
• W2069732020 hasRelatedWork W2070384939 @default.
• W2069732020 hasRelatedWork W2079458286 @default.
• W2069732020 hasRelatedWork W2088515954 @default.
• W2069732020 hasRelatedWork W2092684862 @default.
• W2069732020 hasRelatedWork W2194153028 @default.
• W2069732020 hasRelatedWork W2402806859 @default.
• W2069732020 hasRelatedWork W389083620 @default.
• W2069732020 hasRelatedWork W90113679 @default.
• W2069732020 isParatext "false" @default.
• W2069732020 isRetracted "false" @default.
• W2069732020 magId "2069732020" @default.
• W2069732020 workType "article" @default.