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• W2069751570 abstract "Different assays have been developed in the past years to meet point-of-care diagnostic tests requirements for fast and sensitive quantification and identification of targets. In this paper, we developed the ligation chain reaction (LCR) assay on the Fiber Optic Surface Plasmon Resonance (FO-SPR) platform, which enabled simultaneous quantification and cycle-to-cycle identification of DNA during amplification. The newly developed assay incorporated FO-SPR DNA melting assay, previously developed by our group. This required establishment of several assay parameters, including buffer ionic strength and thermal ramping speed as these parameters both influence the ligation enzyme performance and the hybridization yield of the gold nanoparticles (Au NPs) on the FO-SPR sensor. Quantification and identification of DNA targets was achieved over a wide concentration range with a calibration curve spanning 7 orders of magnitude and LOD of 13.75 fM. Moreover, the FO-SPR LCR assay could discriminate single nucleotide polymorphism (SNPs) without any post reaction analysis, featuring thus all the essential requirements of POC tests." @default.
• W2069751570 created "2016-06-24" @default.
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• W2069751570 date "2015-05-01" @default.
• W2069751570 modified "2023-09-23" @default.
• W2069751570 title "Real-time ligation chain reaction for DNA quantification and identification on the FO-SPR" @default.
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• W2069751570 doi "https://doi.org/10.1016/j.bios.2014.08.067" @default.
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