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• W2069759058 abstract "Single-chain observations on the conformational change of giant DNA (T4 DNA) molecules were performed using fluorescence microscopy at different values of pH in the presence of spermine. Individual DNA molecules undergo a large discrete change, or all-or-none transition, in conformation from a folded compact state to an unfolded coil state with an increase in pH. This abrupt unfolding of DNA with an increase in pH is attributed to a decrease in the concentration of the tetravalent form in spermine [SPM4+]. We propose a scheme for the folding transition of single DNAs, where the manner of spermine binding changes dramatically from weak loose binding in the elongated coil state to strong tight binding in the folded compact state. We discuss the hierarchical nature of the transition, i.e. cooperative continuous change on the ensemble vs. all-or-none switching on individual DNAs." @default.
• W2069759058 created "2016-06-24" @default.
• W2069759058 creator A5010168885 @default.
• W2069759058 creator A5070416808 @default.
• W2069759058 date "2002-09-01" @default.
• W2069759058 modified "2023-10-18" @default.
• W2069759058 title "Proton concentration (pH) switches the higher-order structure of DNA in the presence of spermine" @default.
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• W2069759058 doi "https://doi.org/10.1016/s0301-4622(02)00110-2" @default.
• W2069759058 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12223238" @default.
• W2069759058 hasPublicationYear "2002" @default.
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