Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069759131> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2069759131 endingPage "1297" @default.
- W2069759131 startingPage "1293" @default.
- W2069759131 abstract "About a 16-fold rise in acid phosphatase (EC 3.1.3.2) activity was observed during the early stages of germination of cotton embryos. Administration of cyclobeximide to the germinating embryos significantly blocked the enhancement of acid phosphatase activity. This indicated that translational activity was essential for the induction of enzyme activity. Conclusive proof for the de novo synthesis of the enzyme was obtained by showing the incorporation of 35S from 35SO2−4 into the cysteine residues of the purified acid phosphatase. The enzyme was purified (1046-fold) to electrophoretic homogeneity by ammonium sulphate fractionation, CM-Sephadex C-50 and affinity chromatography on concanavalin A-Agarose. PAGE gave two isozyme bands. The M, of the phosphatase was 200 k as determined by molecular sieving on Sephadex G-200. SDS-PAGE of acid phosphatase revealed a single band of M 55 k. Thus the native enzyme is a tetramer of four identical subunits. The Km of the enzyme with p-nitrophenyl phosphate was 0.5 mM. Optimal enzyme activity was observed at pH 5.0, using p-nitrophenyl phosphate as substrate. The enzyme activity remained linear for 105 min at 37° and was proportional to the concentration of protein within the range 0.6–2.4 μg." @default.
- W2069759131 created "2016-06-24" @default.
- W2069759131 creator A5086984685 @default.
- W2069759131 creator A5087554135 @default.
- W2069759131 date "1987-04-01" @default.
- W2069759131 modified "2023-09-27" @default.
- W2069759131 title "Induction of acid phosphatase in cotton seedlings: Enzyme purification, subunit structure and kinetic properties" @default.
- W2069759131 cites W109384325 @default.
- W2069759131 cites W1594973996 @default.
- W2069759131 cites W1757518583 @default.
- W2069759131 cites W1775749144 @default.
- W2069759131 cites W1969135423 @default.
- W2069759131 cites W1970573899 @default.
- W2069759131 cites W1972986105 @default.
- W2069759131 cites W1974943329 @default.
- W2069759131 cites W1975777610 @default.
- W2069759131 cites W1996148615 @default.
- W2069759131 cites W2028544435 @default.
- W2069759131 cites W2031683336 @default.
- W2069759131 cites W2045028985 @default.
- W2069759131 cites W2047066744 @default.
- W2069759131 cites W2062000380 @default.
- W2069759131 cites W2076636619 @default.
- W2069759131 cites W2083531952 @default.
- W2069759131 cites W2100837269 @default.
- W2069759131 cites W2166867387 @default.
- W2069759131 cites W2303011402 @default.
- W2069759131 cites W4238472358 @default.
- W2069759131 cites W62139898 @default.
- W2069759131 doi "https://doi.org/10.1016/s0031-9422(00)81797-5" @default.
- W2069759131 hasPublicationYear "1987" @default.
- W2069759131 type Work @default.
- W2069759131 sameAs 2069759131 @default.
- W2069759131 citedByCount "30" @default.
- W2069759131 countsByYear W20697591312012 @default.
- W2069759131 countsByYear W20697591312014 @default.
- W2069759131 countsByYear W20697591312021 @default.
- W2069759131 crossrefType "journal-article" @default.
- W2069759131 hasAuthorship W2069759131A5086984685 @default.
- W2069759131 hasAuthorship W2069759131A5087554135 @default.
- W2069759131 hasConcept C119795356 @default.
- W2069759131 hasConcept C160160445 @default.
- W2069759131 hasConcept C163994747 @default.
- W2069759131 hasConcept C178666793 @default.
- W2069759131 hasConcept C181199279 @default.
- W2069759131 hasConcept C185592680 @default.
- W2069759131 hasConcept C198684833 @default.
- W2069759131 hasConcept C19869320 @default.
- W2069759131 hasConcept C202751555 @default.
- W2069759131 hasConcept C21547065 @default.
- W2069759131 hasConcept C2778886173 @default.
- W2069759131 hasConcept C28212737 @default.
- W2069759131 hasConcept C43617362 @default.
- W2069759131 hasConcept C55493867 @default.
- W2069759131 hasConceptScore W2069759131C119795356 @default.
- W2069759131 hasConceptScore W2069759131C160160445 @default.
- W2069759131 hasConceptScore W2069759131C163994747 @default.
- W2069759131 hasConceptScore W2069759131C178666793 @default.
- W2069759131 hasConceptScore W2069759131C181199279 @default.
- W2069759131 hasConceptScore W2069759131C185592680 @default.
- W2069759131 hasConceptScore W2069759131C198684833 @default.
- W2069759131 hasConceptScore W2069759131C19869320 @default.
- W2069759131 hasConceptScore W2069759131C202751555 @default.
- W2069759131 hasConceptScore W2069759131C21547065 @default.
- W2069759131 hasConceptScore W2069759131C2778886173 @default.
- W2069759131 hasConceptScore W2069759131C28212737 @default.
- W2069759131 hasConceptScore W2069759131C43617362 @default.
- W2069759131 hasConceptScore W2069759131C55493867 @default.
- W2069759131 hasIssue "5" @default.
- W2069759131 hasLocation W20697591311 @default.
- W2069759131 hasOpenAccess W2069759131 @default.
- W2069759131 hasPrimaryLocation W20697591311 @default.
- W2069759131 hasRelatedWork W1992430433 @default.
- W2069759131 hasRelatedWork W2018814533 @default.
- W2069759131 hasRelatedWork W2024782539 @default.
- W2069759131 hasRelatedWork W2046181135 @default.
- W2069759131 hasRelatedWork W2069759131 @default.
- W2069759131 hasRelatedWork W2083531952 @default.
- W2069759131 hasRelatedWork W2087616663 @default.
- W2069759131 hasRelatedWork W2114819079 @default.
- W2069759131 hasRelatedWork W2135379436 @default.
- W2069759131 hasRelatedWork W2437002429 @default.
- W2069759131 hasVolume "26" @default.
- W2069759131 isParatext "false" @default.
- W2069759131 isRetracted "false" @default.
- W2069759131 magId "2069759131" @default.
- W2069759131 workType "article" @default.