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- W2069778030 abstract "Four commercial antigen extracts of Aspergillus fumigatus were evaluated for use in a rapid enzyme-linked immunosorbent assay (ELISA) for anti-A. fumigatus IgG. Initial binding of both somatic and culture filtrate preparations to a polyvinyl chloride solid phase was concentration dependent and increased with incubation time. Antigen binding to the solid phase was reproducible. Binding of A. fumigatus precipitin-positive serum to bound antigen was rapid. All four A. fumigatus antigens demonstrated similar dose-response curves when tested against pooled sera containing a high titre of A. fumigatus antibodies. Detectable activity in precipitin test-negative sera decreased rapidly with dilution. All the antigen preparations were found to be suitable for ELISA procedures and permit the rapid determination of IgG antibodies to A. fumigatus." @default.
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- W2069778030 date "1984-01-01" @default.
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- W2069778030 title "Antigen and antibody attachment in ELISA for Aspergillus fumigatus IgG antibodies" @default.
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- W2069778030 doi "https://doi.org/10.1016/0022-1759(84)90255-2" @default.
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