Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069793609> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2069793609 abstract "We present an efficient method for the production of N-acetyl-L-phosphinothricin (N-AcPt) from commercial DL-phosphinothricin (DL-PPT) by organic acetylation for use as a negative selection agent (NSA) that induces cell death in argE transgenic rice. DL-PPT was efficiently converted into N-AcPt with tetrahydrofuran (THF) and acetic anhydride (Ac2O). Chemical changes were confirmed using NMR and ATR-FTIR analyses. DL-PPT was toxic but N-AcPt did not show cytotoxic effects on leaf discs or seed germination of wild-type rice. Conversely, in argE-hpt transgenic rice, non-toxic N-AcPt showed the negative selection (NS) effect by inducing cell destruction in leaf discs and restricting seed germination. For inducing NS, ≥0.1 mg ml(-1) and ≥0.5 mg ml(-1) of N-AcPt were effective in leaf and seed assays, respectively. Further, the NS effect occurred faster in the leaf assay compared with the seed germination assay, again indicating the leaf assay was a more sensitive indicator of N-AcPt as an NSA to argE transgenic rice than the seed germination assay. This negative selection approach could be useful for the development of selectable marker free transgenic plants in the economically important monocot species and its commercialization for multiple gene transformation." @default.
- W2069793609 created "2016-06-24" @default.
- W2069793609 creator A5024810045 @default.
- W2069793609 creator A5063244924 @default.
- W2069793609 date "2013-11-01" @default.
- W2069793609 modified "2023-09-24" @default.
- W2069793609 title "An efficient method for organic acetylation and use of dl-phosphinothricin as a negative selection agent in argE transgenic rice" @default.
- W2069793609 cites W1600209892 @default.
- W2069793609 cites W1666250587 @default.
- W2069793609 cites W1973751673 @default.
- W2069793609 cites W1998793515 @default.
- W2069793609 cites W2003231662 @default.
- W2069793609 cites W2008054069 @default.
- W2069793609 cites W2014230904 @default.
- W2069793609 cites W2014361819 @default.
- W2069793609 cites W2014736790 @default.
- W2069793609 cites W2018494740 @default.
- W2069793609 cites W2039878852 @default.
- W2069793609 cites W2041820866 @default.
- W2069793609 cites W2070549081 @default.
- W2069793609 cites W2072449416 @default.
- W2069793609 cites W2075051663 @default.
- W2069793609 cites W2085217535 @default.
- W2069793609 cites W2094946613 @default.
- W2069793609 cites W2116736320 @default.
- W2069793609 cites W2131002926 @default.
- W2069793609 cites W2135599428 @default.
- W2069793609 cites W2140674122 @default.
- W2069793609 cites W2143664231 @default.
- W2069793609 cites W2154893268 @default.
- W2069793609 cites W2158752477 @default.
- W2069793609 cites W2166396989 @default.
- W2069793609 cites W2175442085 @default.
- W2069793609 cites W2325885552 @default.
- W2069793609 cites W2522489013 @default.
- W2069793609 cites W3165825451 @default.
- W2069793609 doi "https://doi.org/10.1016/j.bbrc.2013.10.048" @default.
- W2069793609 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24141117" @default.
- W2069793609 hasPublicationYear "2013" @default.
- W2069793609 type Work @default.
- W2069793609 sameAs 2069793609 @default.
- W2069793609 citedByCount "1" @default.
- W2069793609 countsByYear W20697936092019 @default.
- W2069793609 crossrefType "journal-article" @default.
- W2069793609 hasAuthorship W2069793609A5024810045 @default.
- W2069793609 hasAuthorship W2069793609A5063244924 @default.
- W2069793609 hasConcept C100701293 @default.
- W2069793609 hasConcept C102230213 @default.
- W2069793609 hasConcept C104317684 @default.
- W2069793609 hasConcept C105409712 @default.
- W2069793609 hasConcept C119157956 @default.
- W2069793609 hasConcept C122172344 @default.
- W2069793609 hasConcept C177955015 @default.
- W2069793609 hasConcept C185592680 @default.
- W2069793609 hasConcept C204241405 @default.
- W2069793609 hasConcept C55493867 @default.
- W2069793609 hasConcept C59822182 @default.
- W2069793609 hasConcept C86803240 @default.
- W2069793609 hasConceptScore W2069793609C100701293 @default.
- W2069793609 hasConceptScore W2069793609C102230213 @default.
- W2069793609 hasConceptScore W2069793609C104317684 @default.
- W2069793609 hasConceptScore W2069793609C105409712 @default.
- W2069793609 hasConceptScore W2069793609C119157956 @default.
- W2069793609 hasConceptScore W2069793609C122172344 @default.
- W2069793609 hasConceptScore W2069793609C177955015 @default.
- W2069793609 hasConceptScore W2069793609C185592680 @default.
- W2069793609 hasConceptScore W2069793609C204241405 @default.
- W2069793609 hasConceptScore W2069793609C55493867 @default.
- W2069793609 hasConceptScore W2069793609C59822182 @default.
- W2069793609 hasConceptScore W2069793609C86803240 @default.
- W2069793609 hasFunder F4320321213 @default.
- W2069793609 hasLocation W20697936091 @default.
- W2069793609 hasLocation W20697936092 @default.
- W2069793609 hasOpenAccess W2069793609 @default.
- W2069793609 hasPrimaryLocation W20697936091 @default.
- W2069793609 hasRelatedWork W1497504526 @default.
- W2069793609 hasRelatedWork W1975403121 @default.
- W2069793609 hasRelatedWork W2028807400 @default.
- W2069793609 hasRelatedWork W2092093697 @default.
- W2069793609 hasRelatedWork W2139091484 @default.
- W2069793609 hasRelatedWork W2153186861 @default.
- W2069793609 hasRelatedWork W2182864544 @default.
- W2069793609 hasRelatedWork W2348594442 @default.
- W2069793609 hasRelatedWork W2392000336 @default.
- W2069793609 hasRelatedWork W84467322 @default.
- W2069793609 isParatext "false" @default.
- W2069793609 isRetracted "false" @default.
- W2069793609 magId "2069793609" @default.
- W2069793609 workType "article" @default.