Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069816659> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2069816659 endingPage "7032" @default.
- W2069816659 startingPage "7024" @default.
- W2069816659 abstract "Columns switch recycling size exclusion chromatography (csrSEC) was proposed to achieve high resolution protein separation with good biocompatibility. Proteins were firstly separated by two serially coupled SEC columns, and fractions were in sequence switched back to the first column by two-position valves for further separation in terms of close-loop recycling until satisfactory resolution was achieved. Compared to SEC, the separation window was broadened by increasing column length via cycling without further increase on back pressure. Compared to recycling SEC (rSEC), the overtaking of later eluted components by early eluted ones after several cycles could be avoided for complex sample analysis, by parking fractions in the second SEC column before transferred in turn back to the first one for cycling ordinally. In our experiments, the baseline separation of five proteins with molecular weight ranging from 10 kDa to 80kDa was achieved by csrSEC. Furthermore, a multidimensional csrSEC-RPLC platform was constructed, and peak capacity up to 3600 was obtained for protein separation. All these results demonstrated that csrSEC is a promising protein separation mode with good biocompatibility, broadened separation window and improved resolution." @default.
- W2069816659 created "2016-06-24" @default.
- W2069816659 creator A5005783685 @default.
- W2069816659 creator A5007869766 @default.
- W2069816659 creator A5032225807 @default.
- W2069816659 creator A5060264631 @default.
- W2069816659 creator A5060842938 @default.
- W2069816659 date "2009-10-01" @default.
- W2069816659 modified "2023-10-10" @default.
- W2069816659 title "Columns switch recycling size exclusion chromatography for high resolution protein separation" @default.
- W2069816659 cites W1973076818 @default.
- W2069816659 cites W1988098839 @default.
- W2069816659 cites W1995408073 @default.
- W2069816659 cites W1997774271 @default.
- W2069816659 cites W1998415830 @default.
- W2069816659 cites W2007286790 @default.
- W2069816659 cites W2016316557 @default.
- W2069816659 cites W2018708336 @default.
- W2069816659 cites W2022595374 @default.
- W2069816659 cites W2029502931 @default.
- W2069816659 cites W2031633859 @default.
- W2069816659 cites W2039946243 @default.
- W2069816659 cites W2043050470 @default.
- W2069816659 cites W2062438096 @default.
- W2069816659 cites W2075116108 @default.
- W2069816659 cites W2077644009 @default.
- W2069816659 cites W2087461343 @default.
- W2069816659 cites W2089142914 @default.
- W2069816659 cites W2095448912 @default.
- W2069816659 cites W2112991229 @default.
- W2069816659 cites W2113242836 @default.
- W2069816659 cites W2151002547 @default.
- W2069816659 cites W2164036543 @default.
- W2069816659 doi "https://doi.org/10.1016/j.chroma.2009.08.065" @default.
- W2069816659 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19758593" @default.
- W2069816659 hasPublicationYear "2009" @default.
- W2069816659 type Work @default.
- W2069816659 sameAs 2069816659 @default.
- W2069816659 citedByCount "16" @default.
- W2069816659 countsByYear W20698166592012 @default.
- W2069816659 countsByYear W20698166592013 @default.
- W2069816659 countsByYear W20698166592014 @default.
- W2069816659 countsByYear W20698166592016 @default.
- W2069816659 countsByYear W20698166592018 @default.
- W2069816659 countsByYear W20698166592020 @default.
- W2069816659 countsByYear W20698166592022 @default.
- W2069816659 crossrefType "journal-article" @default.
- W2069816659 hasAuthorship W2069816659A5005783685 @default.
- W2069816659 hasAuthorship W2069816659A5007869766 @default.
- W2069816659 hasAuthorship W2069816659A5032225807 @default.
- W2069816659 hasAuthorship W2069816659A5060264631 @default.
- W2069816659 hasAuthorship W2069816659A5060842938 @default.
- W2069816659 hasConcept C113196181 @default.
- W2069816659 hasConcept C127313418 @default.
- W2069816659 hasConcept C138268822 @default.
- W2069816659 hasConcept C154945302 @default.
- W2069816659 hasConcept C181199279 @default.
- W2069816659 hasConcept C185592680 @default.
- W2069816659 hasConcept C193363816 @default.
- W2069816659 hasConcept C3020199158 @default.
- W2069816659 hasConcept C41008148 @default.
- W2069816659 hasConcept C43617362 @default.
- W2069816659 hasConcept C55493867 @default.
- W2069816659 hasConcept C62649853 @default.
- W2069816659 hasConcept C73826308 @default.
- W2069816659 hasConceptScore W2069816659C113196181 @default.
- W2069816659 hasConceptScore W2069816659C127313418 @default.
- W2069816659 hasConceptScore W2069816659C138268822 @default.
- W2069816659 hasConceptScore W2069816659C154945302 @default.
- W2069816659 hasConceptScore W2069816659C181199279 @default.
- W2069816659 hasConceptScore W2069816659C185592680 @default.
- W2069816659 hasConceptScore W2069816659C193363816 @default.
- W2069816659 hasConceptScore W2069816659C3020199158 @default.
- W2069816659 hasConceptScore W2069816659C41008148 @default.
- W2069816659 hasConceptScore W2069816659C43617362 @default.
- W2069816659 hasConceptScore W2069816659C55493867 @default.
- W2069816659 hasConceptScore W2069816659C62649853 @default.
- W2069816659 hasConceptScore W2069816659C73826308 @default.
- W2069816659 hasIssue "42" @default.
- W2069816659 hasLocation W20698166591 @default.
- W2069816659 hasLocation W20698166592 @default.
- W2069816659 hasOpenAccess W2069816659 @default.
- W2069816659 hasPrimaryLocation W20698166591 @default.
- W2069816659 hasRelatedWork W2005165146 @default.
- W2069816659 hasRelatedWork W2026395478 @default.
- W2069816659 hasRelatedWork W2026912780 @default.
- W2069816659 hasRelatedWork W2054646844 @default.
- W2069816659 hasRelatedWork W2151131743 @default.
- W2069816659 hasRelatedWork W2311474148 @default.
- W2069816659 hasRelatedWork W2318291238 @default.
- W2069816659 hasRelatedWork W3190051883 @default.
- W2069816659 hasRelatedWork W4212954839 @default.
- W2069816659 hasRelatedWork W4248898387 @default.
- W2069816659 hasVolume "1216" @default.
- W2069816659 isParatext "false" @default.
- W2069816659 isRetracted "false" @default.
- W2069816659 magId "2069816659" @default.
- W2069816659 workType "article" @default.