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- W2069843912 abstract "We report a simplified micropatterning method for the straight-line extension of the neurites of cultured neurons. We prepared a poly-D-lysine (PDL)-patterned surface using a polydimethylsiloxane microfluidic stamp. Hippocampal neurons were cultured on the PDL-bound substrate with the stamp removed, allowing for conventional cell seeding and detailed optical observation without fluorescent label. Cultured neurons elongated neurites along straight lines at the single-cell level and displayed spontaneous firing as detected by time-lapse imaging and Ca(2+) imaging." @default.
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- W2069843912 date "2013-02-01" @default.
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- W2069843912 title "A Simplified Micropatterning Method for Straight-line Neurite Extension of Cultured Hippocampal Neurons" @default.
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- W2069843912 doi "https://doi.org/10.2116/analsci.29.263" @default.
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