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• W2069852183 startingPage "435" @default.
• W2069852183 abstract "Based on in vivo selection of effective suppressor tRNAs from two different combinatorial gene libraries in which several nucleotides in the D and T-loops were randomized, we show that the position of the reverse-Hoogsteen base-pair in the T-loop, normally formed between nucleotides 54-58, co-varies with the length of the D-domain. When the D-domain has the normal length, the position of the reverse-Hoogsteen base-pair in the T-loop is always such that it allocates two unpaired nucleotides 59-60 for the bulge that fills the space between the D and T-domains. However, when the D-domain becomes shorter, the position of the reverse-Hoogsteen base-pair changes in the way that more nucleotides are now allocated to the T-loop bulge, so that the total length of the D-domain and of the bulge remains the same. Such compensation guarantees that in all tRNAs, the D and T-domains are always juxtaposed in the standard way. It also demonstrates the major role of the two T-loop elements, the bulge and the reverse-Hoogsteen base-pair, in the formation of the canonical tRNA L-shape." @default.
• W2069852183 created "2016-06-24" @default.
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• W2069852183 creator A5053432778 @default.
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• W2069852183 date "2004-07-01" @default.
• W2069852183 modified "2023-10-14" @default.
• W2069852183 title "Key Elements in Maintenance of the tRNA L-shape" @default.
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• W2069852183 doi "https://doi.org/10.1016/j.jmb.2004.05.002" @default.
• W2069852183 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15210345" @default.
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