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• W2069856593 abstract "The effect of Rp diastereoisomer of adenosine 3′,5′-cyclic monophosphothioate (Rp-cAMPS) on relaxation elicited by histamine (1–100 μM), forskolin (1–60 μM), papaverine (1–100 μM), vinpocetine (1–100 μM), rolipram (0.1–1 mM), Sp-cAMPS (10–300 μM), 8-BrcAMP (10 μM-1 mM) and 8-BrcGMP (3 μM-1 mM) of the previous vanadate-induced contraction was assayed. The effect of Rp-cAMPS on the relaxing effect produced by forskolin, papaverine, vinpocetine, rolipram, Sp-cAMPS and 8-BrcAMP in KCl-induced tonic contraction was also assayed. Histamine, forskolin, papaverine, rolipram, Sp-cAMPS, 8-BrcAMP and 8-BrcGMP, but not vinpocetine, relaxed the vanadate-induced contractions in rat uterus incubated in medium lacking calcium plus EDTA in a concentration-dependent way. Rp-cAMPS (1–300 μM) had no effect on vanadate contraction. However, it antagonized the relaxation elicited by histamine and papaverine, but not that of forskolin, rolipram, Sp-cAMPS, 8-BrcAMP and 8-BrcGMP. Forskolin, papaverine, vinpocetine, rolipram and 8-BrcAMP, but not Sp-cAMPS, relaxed the KCl-induced contraction. Rp-cAMPS antagonized the relaxation elicited by forskolin, papaverine and vinpocetine, but not that of rolipram and 8-BrcAMP. Our results suggest that: a) Rp-cAMPS is an effective PKA inhibitor that could be used to study the involvement of cAMP on drug-induced response in smooth muscle, and b) the effects of Sp-cAMPS, 8-BrcAMP and rolipram were independent of the activation of protein kinases." @default.
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• W2069856593 date "1997-07-01" @default.
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• W2069856593 title "Effect of Rp diastereoisomer of adenosine 3′,5′ cyclic-monophosphothioate on the cAMP-dependent relaxation of smooth muscle" @default.
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• W2069856593 doi "https://doi.org/10.1016/s0024-3205(97)00589-4" @default.
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