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• W2069858503 abstract "Abstract A highly sensitive and convenient assay for epoxide hydrolase, which uses a tritiated endogenous steroid epoxide (16α, 17α-[17-3H]epoxyandrost-4-en-3-one) as substrate, is described. The method involves a simple extraction procedure for separating the product of the enzymatic reaction (16β,17α-dihydroxyandrost-4-en-3-one) from the incubation mixture and unreacted epoxide. Quantitation of epoxide hydrolase activity is achieved by scintillation photometry of the tritium-labeled diol product. The assay is very sensitive because of (a) the low blank value (0.2% of added radioactivity, because nonenzymaic hydrolysis of the substrate could not be detected and a very low amount of radioactive substrate and/or impurities are left in the final sample) and (b) the V of the enzyme toward androstene oxide (55 nmol diol/min/mg protein) and the resulting low amount of protein required per incubation. These features and the simplicity of the method make this assay widely applicable and valuable for the determination of epoxide hydrolase activity in tissue samples of low mass or low activity. Additionally, the assay is very important for the investigation of the physiological role of epoxide hydrolase, since it uses an endogenous substrate of the enzyme." @default.
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• W2069858503 date "1981-10-01" @default.
• W2069858503 modified "2023-10-01" @default.
• W2069858503 title "A highly sensitive assay for epoxide hydrolase using an endogenous epoxide as substrate: 16α, 17α-epoxyandrost-4-en-3-one" @default.
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• W2069858503 doi "https://doi.org/10.1016/0003-2697(81)90715-6" @default.
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