Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069889949> ?p ?o ?g. }
- W2069889949 endingPage "e48067" @default.
- W2069889949 startingPage "e48067" @default.
- W2069889949 abstract "Cell transplantation for regenerative medicine has become an appealing therapeutic method; however, stem and progenitor cells are not always freshly available. Cryopreservation offers a way to freeze cells as they are generated, for storage and transport until required for therapy. This study was performed to assess the feasibility of cryopreserving peripheral blood mononuclear cells (PBMCs) for the subsequent in vitro generation of their derived therapeutic population, circulating angiogenic cells (CACs).PBMCs were isolated from healthy human donors. Freshly isolated cells were either analyzed immediately or cryopreserved in media containing 6% plasma serum and 5% dimethyl sulfoxide. PBMCs were thawed after being frozen for 1 (early thaw) or 28 (late thaw) days and analyzed, or cultured for 4 days to generate CACs. Analysis of the cells consisted of flow cytometry for viability and phenotype, as well as functional assays for their adhesion and migration potential, cytokine secretion, and in vivo angiogenic potential.The viability of PBMCs and CACs as well as their adhesion and migration properties did not differ greatly after cryopreservation. Phenotypic changes did occur in PBMCs and to a lesser extent in CACs after freezing; however the potent CD34(+)VEGFR2(+)CD133(+) population remained unaffected. The derived CACs, while exhibiting changes in inflammatory cytokine secretion, showed no changes in the secretion of important regenerative and chemotactic cytokines, nor in their ability to restore perfusion in ischemic muscle.Overall, it appears that changes do occur in cryopreserved PBMCs and their generated CACs; however, the CD34(+)VEGFR2(+)CD133(+) progenitor population, the secretion of pro-vasculogenic factors, and the in vivo angiogenic potential of CACs remain unaffected by cryopreservation." @default.
- W2069889949 created "2016-06-24" @default.
- W2069889949 creator A5024493344 @default.
- W2069889949 creator A5026408371 @default.
- W2069889949 creator A5033701201 @default.
- W2069889949 creator A5045750738 @default.
- W2069889949 creator A5050591399 @default.
- W2069889949 creator A5062455569 @default.
- W2069889949 creator A5090376471 @default.
- W2069889949 date "2012-10-25" @default.
- W2069889949 modified "2023-09-23" @default.
- W2069889949 title "Circulating Angiogenic Cells can be Derived from Cryopreserved Peripheral Blood Mononuclear Cells" @default.
- W2069889949 cites W1492387747 @default.
- W2069889949 cites W1502896205 @default.
- W2069889949 cites W1705661556 @default.
- W2069889949 cites W1964157625 @default.
- W2069889949 cites W1964257992 @default.
- W2069889949 cites W1973352757 @default.
- W2069889949 cites W1976291192 @default.
- W2069889949 cites W1978238305 @default.
- W2069889949 cites W1981769649 @default.
- W2069889949 cites W1982651263 @default.
- W2069889949 cites W1987111733 @default.
- W2069889949 cites W1997315475 @default.
- W2069889949 cites W1999579644 @default.
- W2069889949 cites W2008062232 @default.
- W2069889949 cites W2011527383 @default.
- W2069889949 cites W2016584479 @default.
- W2069889949 cites W2027329004 @default.
- W2069889949 cites W2034463755 @default.
- W2069889949 cites W2038603463 @default.
- W2069889949 cites W2041784744 @default.
- W2069889949 cites W2059667214 @default.
- W2069889949 cites W2060902798 @default.
- W2069889949 cites W2064817281 @default.
- W2069889949 cites W2067296853 @default.
- W2069889949 cites W2069467875 @default.
- W2069889949 cites W2080836044 @default.
- W2069889949 cites W2088509353 @default.
- W2069889949 cites W2093486554 @default.
- W2069889949 cites W2096910845 @default.
- W2069889949 cites W2098767125 @default.
- W2069889949 cites W2098802709 @default.
- W2069889949 cites W2105941005 @default.
- W2069889949 cites W2108285596 @default.
- W2069889949 cites W2108875285 @default.
- W2069889949 cites W2110667326 @default.
- W2069889949 cites W2112633556 @default.
- W2069889949 cites W2136341324 @default.
- W2069889949 cites W2142017954 @default.
- W2069889949 cites W2142634601 @default.
- W2069889949 cites W2145639716 @default.
- W2069889949 cites W2154353368 @default.
- W2069889949 cites W2170726221 @default.
- W2069889949 cites W2315700954 @default.
- W2069889949 cites W4235280245 @default.
- W2069889949 cites W4236554944 @default.
- W2069889949 cites W4313422280 @default.
- W2069889949 doi "https://doi.org/10.1371/journal.pone.0048067" @default.
- W2069889949 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3483876" @default.
- W2069889949 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23133548" @default.
- W2069889949 hasPublicationYear "2012" @default.
- W2069889949 type Work @default.
- W2069889949 sameAs 2069889949 @default.
- W2069889949 citedByCount "7" @default.
- W2069889949 countsByYear W20698899492013 @default.
- W2069889949 countsByYear W20698899492014 @default.
- W2069889949 countsByYear W20698899492016 @default.
- W2069889949 countsByYear W20698899492017 @default.
- W2069889949 countsByYear W20698899492023 @default.
- W2069889949 crossrefType "journal-article" @default.
- W2069889949 hasAuthorship W2069889949A5024493344 @default.
- W2069889949 hasAuthorship W2069889949A5026408371 @default.
- W2069889949 hasAuthorship W2069889949A5033701201 @default.
- W2069889949 hasAuthorship W2069889949A5045750738 @default.
- W2069889949 hasAuthorship W2069889949A5050591399 @default.
- W2069889949 hasAuthorship W2069889949A5062455569 @default.
- W2069889949 hasAuthorship W2069889949A5090376471 @default.
- W2069889949 hasBestOaLocation W20698899491 @default.
- W2069889949 hasConcept C10205521 @default.
- W2069889949 hasConcept C137061746 @default.
- W2069889949 hasConcept C150903083 @default.
- W2069889949 hasConcept C16685009 @default.
- W2069889949 hasConcept C179933525 @default.
- W2069889949 hasConcept C185592680 @default.
- W2069889949 hasConcept C196843134 @default.
- W2069889949 hasConcept C201750760 @default.
- W2069889949 hasConcept C202751555 @default.
- W2069889949 hasConcept C203014093 @default.
- W2069889949 hasConcept C207001950 @default.
- W2069889949 hasConcept C26291073 @default.
- W2069889949 hasConcept C2778690821 @default.
- W2069889949 hasConcept C28328180 @default.
- W2069889949 hasConcept C2908647359 @default.
- W2069889949 hasConcept C55493867 @default.
- W2069889949 hasConcept C71924100 @default.
- W2069889949 hasConcept C86803240 @default.
- W2069889949 hasConcept C95444343 @default.