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- W2069897196 abstract "Recombinant f1 phages carrying a shuttle vector pKA1M for expression of blasticidin S deaminase were introduced into monkey COS7 cells by mixing with dioctadecylamidoglycylspermine (DOGS). Blasticidin S selection resulted in the detectable growth of resistant colonies within a week. The transfection efficiency depended on the amounts of the phage and DOGS, their ratio, and the time during which the cells were incubated with the phage/DOGS mixture. This method requires only several microliters of an Escherichia coli culture medium containing recombinant f1 phage particles and is applicable to various cell lines including mouse NIH/3T3, chinese hamster CHO-K1, and human HT-1080." @default.
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- W2069897196 title "Recombinant f1 Phage-Mediated Transfection of Mammalian Cells Using Lipopolyamine Technique" @default.
- W2069897196 doi "https://doi.org/10.1006/abio.1994.1557" @default.
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