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- W2069918977 abstract "Arabidopsis phosphatidylinositol phosphate (PtdInsP) kinase 10 (AtPIPK10; At4g01190) is shown to be a functional enzyme of the subfamily A, type I AtPtdInsP kinases. It is biochemically distinct from AtPIPK1 (At1g21980), the only other previously characterized AtPtdInsP kinase which is of the B subfamily. AtPIPK10 has the same K m, but a 10-fold lower V max than AtPIPK1 and it is insensitive to phosphatidic acid. AtPIPK10 transcript is most abundant in inflorescence stalks and flowers, whereas AtPIPK1 transcript is present in all tissues. Comparative analysis of recombinant AtPIPK10 and AtPIPK1 with recombinant HsPIPKIα reveals that the Arabidopsis enzymes have roughly 200- and 20-fold lower V max/K m, respectively. These data reveal one explanation for the longstanding mystery of the relatively low phosphatidylinositol-(4,5)-bisphosphate:phosphatidylinositol-4-phosphate ratio in terrestrial plants." @default.
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- W2069918977 date "2005-05-31" @default.
- W2069918977 modified "2023-09-27" @default.
- W2069918977 title "Characterization and comparative analysis of<i>Arabidopsis</i>phosphatidylinositol phosphate 5-kinase 10 reveals differences in<i>Arabidopsis</i>and human phosphatidylinositol phosphate kinases" @default.
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- W2069918977 doi "https://doi.org/10.1016/j.febslet.2005.05.018" @default.
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