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- W2069932008 abstract "Class II histocompatibility antigens were assessed by complement-dependent cytotoxicity on B enriched mononuclear cells and on purified monocytes isolated from the peripheral blood of renal patients before and after transplantation. After transplantation, a significant decrease of detection of DR alloantigens (cytotoxicity score before transplantation 3 × 0.1; after transplantation 0.95 × 0.1; p < 0.001) was found. A similar defect was observed both on B enriched frozen cells and on purified monocytes isolated during the post-transplant period. Nevertheless, antigens could be adequately detected by a sensitive microabsorption method and by a procedure involving preincubation of mononuclear cells at 37°C for 3 h followed by B cells enrichment by rosetting. The 37°C induced reexpression could be inhibited by 10 μg/ml cycloheximide and by 50 μM monensin. No difference was found in the magnitude either of decreased expression or of temperature-induced reexpression between patients treated by CyS-Pred and those receiving Aza-Pred. It is concluded that transplanted recipients disclose a reversible defect of the expression of class II histocompatibility antigens probably related to perturbation of various steps of intracellular biosynthesis and traffic of these molecules." @default.
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- W2069932008 date "2008-12-11" @default.
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- W2069932008 title "Factors involved in decreased detection of class II histocompatibility antigens on blood mononuclear cells from transplanted patients receiving immunosuppressive drugs" @default.
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- W2069932008 doi "https://doi.org/10.1111/j.1399-0039.1986.tb00469.x" @default.
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