Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069949043> ?p ?o ?g. }
- W2069949043 endingPage "150" @default.
- W2069949043 startingPage "143" @default.
- W2069949043 abstract "A two-subunit (alphabeta) form of dissimilatory nitrate reductase from Pseudomonas stutzeri strain ZoBell was separated from the membrane-residing gamma-subunit by a heat solubilization step. Here we present an optimized purification protocol leading to a soluble alphabeta form with high specific activity (70 U/mg). The soluble form has the stoichiometry alpha(1)beta(1) consisting of the 130 kDa alpha-subunit and the 58 kDa beta-subunit. We did not observe any proteolytic cleavage in the course of the heat solubilization. The enzyme is competively inhibited by azide, but not by chlorate. It exhibits a K(M) value of 3.2 mM for nitrate. We compare the enzymatic and electron paramagnetic resonance (EPR) spectroscopic properties of the alphabeta form with the alphabetagamma holoenzyme which resides in the membrane and can be prepared by detergent extraction. The nearly identical EPR spectra for the Mo(V) signal of both enzyme preparations show that the active site is unaffected by the heat step. The factors influencing the binding of the alpha- and beta-subunit to the gamma-subunit are discussed." @default.
- W2069949043 created "2016-06-24" @default.
- W2069949043 creator A5013564230 @default.
- W2069949043 creator A5029271862 @default.
- W2069949043 creator A5032974311 @default.
- W2069949043 creator A5048199197 @default.
- W2069949043 creator A5056507666 @default.
- W2069949043 creator A5084155003 @default.
- W2069949043 date "2002-12-19" @default.
- W2069949043 modified "2023-09-26" @default.
- W2069949043 title "<i>Pseudomonas stutzeri</i> soluble nitrate reductase αβ-subunit is a soluble enzyme with a similar electronic structure at the active site as the inner membrane-bound αβγ holoenzyme" @default.
- W2069949043 cites W1568149732 @default.
- W2069949043 cites W1572812338 @default.
- W2069949043 cites W1605558676 @default.
- W2069949043 cites W1662489613 @default.
- W2069949043 cites W1817977104 @default.
- W2069949043 cites W1924715076 @default.
- W2069949043 cites W1925458372 @default.
- W2069949043 cites W1951321216 @default.
- W2069949043 cites W1956876165 @default.
- W2069949043 cites W1959882277 @default.
- W2069949043 cites W1972388708 @default.
- W2069949043 cites W1975917621 @default.
- W2069949043 cites W1985780751 @default.
- W2069949043 cites W1986321921 @default.
- W2069949043 cites W1997426604 @default.
- W2069949043 cites W2002578376 @default.
- W2069949043 cites W2003280703 @default.
- W2069949043 cites W2027091766 @default.
- W2069949043 cites W2031655005 @default.
- W2069949043 cites W2033459252 @default.
- W2069949043 cites W2034732932 @default.
- W2069949043 cites W2046068872 @default.
- W2069949043 cites W2051956429 @default.
- W2069949043 cites W2056287417 @default.
- W2069949043 cites W2059638582 @default.
- W2069949043 cites W2065434867 @default.
- W2069949043 cites W2070344989 @default.
- W2069949043 cites W2079699706 @default.
- W2069949043 cites W2084068652 @default.
- W2069949043 cites W2086606027 @default.
- W2069949043 cites W2088402240 @default.
- W2069949043 cites W2100837269 @default.
- W2069949043 cites W2101639848 @default.
- W2069949043 cites W2104594638 @default.
- W2069949043 cites W2129973741 @default.
- W2069949043 cites W2145278346 @default.
- W2069949043 cites W2149353640 @default.
- W2069949043 cites W2161870016 @default.
- W2069949043 cites W2251657917 @default.
- W2069949043 cites W2409623748 @default.
- W2069949043 cites W2802786912 @default.
- W2069949043 cites W4293247451 @default.
- W2069949043 cites W47128524 @default.
- W2069949043 doi "https://doi.org/10.1016/s0014-5793(02)03837-1" @default.
- W2069949043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12527376" @default.
- W2069949043 hasPublicationYear "2002" @default.
- W2069949043 type Work @default.
- W2069949043 sameAs 2069949043 @default.
- W2069949043 citedByCount "13" @default.
- W2069949043 countsByYear W20699490432013 @default.
- W2069949043 countsByYear W20699490432014 @default.
- W2069949043 countsByYear W20699490432017 @default.
- W2069949043 crossrefType "journal-article" @default.
- W2069949043 hasAuthorship W2069949043A5013564230 @default.
- W2069949043 hasAuthorship W2069949043A5029271862 @default.
- W2069949043 hasAuthorship W2069949043A5032974311 @default.
- W2069949043 hasAuthorship W2069949043A5048199197 @default.
- W2069949043 hasAuthorship W2069949043A5056507666 @default.
- W2069949043 hasAuthorship W2069949043A5084155003 @default.
- W2069949043 hasBestOaLocation W20699490431 @default.
- W2069949043 hasConcept C104292427 @default.
- W2069949043 hasConcept C104317684 @default.
- W2069949043 hasConcept C121332964 @default.
- W2069949043 hasConcept C181199279 @default.
- W2069949043 hasConcept C185592680 @default.
- W2069949043 hasConcept C187961010 @default.
- W2069949043 hasConcept C2776786877 @default.
- W2069949043 hasConcept C2778710519 @default.
- W2069949043 hasConcept C2910882050 @default.
- W2069949043 hasConcept C46141821 @default.
- W2069949043 hasConcept C523546767 @default.
- W2069949043 hasConcept C54355233 @default.
- W2069949043 hasConcept C55493867 @default.
- W2069949043 hasConcept C71240020 @default.
- W2069949043 hasConcept C86803240 @default.
- W2069949043 hasConceptScore W2069949043C104292427 @default.
- W2069949043 hasConceptScore W2069949043C104317684 @default.
- W2069949043 hasConceptScore W2069949043C121332964 @default.
- W2069949043 hasConceptScore W2069949043C181199279 @default.
- W2069949043 hasConceptScore W2069949043C185592680 @default.
- W2069949043 hasConceptScore W2069949043C187961010 @default.
- W2069949043 hasConceptScore W2069949043C2776786877 @default.
- W2069949043 hasConceptScore W2069949043C2778710519 @default.
- W2069949043 hasConceptScore W2069949043C2910882050 @default.
- W2069949043 hasConceptScore W2069949043C46141821 @default.
- W2069949043 hasConceptScore W2069949043C523546767 @default.
- W2069949043 hasConceptScore W2069949043C54355233 @default.