Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069953162> ?p ?o ?g. }
- W2069953162 endingPage "35" @default.
- W2069953162 startingPage "29" @default.
- W2069953162 abstract "The presence of tumour cells in the circulation may predict disease recurrence and metastasis. To improve on existing methods of cytological or immunocytological detection, we have developed a sensitive and quantitative technique for the detection of carcinoma cells in blood, using the reverse transcriptase polymerase chain reaction (RT-PCR) identifying transcripts of the pancarcinoma-associated tumour marker EGP-2 (KSA or 17-1A antigen). The amount of EGP2 mRNA was quantified using an internal recombinant competitor RNA standard with known concentration and which is both reversely transcribed and co-amplified in the same reaction, allowing for a reliable assessment of the initial amount of EGP2 mRNA in the sample. Calibration studies, seeding blood with MCF-7 breast carcinoma cells, showed that the assay can detect ten tumour cells among 1.0 x 10(6) leucocytes. The PCR assay revealed that normal bone marrow expresses low levels of EGP2 mRNA, although immunocytochemistry with the anti-EGP2 MAb MOC31 could not identify any positively stained cell. Analyses using this RT-PCR assay may prove to have applications to the assessment of circulating tumour cells in clinical samples." @default.
- W2069953162 created "2016-06-24" @default.
- W2069953162 creator A5016904876 @default.
- W2069953162 creator A5019749108 @default.
- W2069953162 creator A5044329653 @default.
- W2069953162 creator A5049603142 @default.
- W2069953162 creator A5051348515 @default.
- W2069953162 creator A5063113967 @default.
- W2069953162 creator A5091383507 @default.
- W2069953162 date "1997-07-01" @default.
- W2069953162 modified "2023-10-09" @default.
- W2069953162 title "A quantitative reverse transcriptase polymerase chain reaction-based assay to detect carcinoma cells in peripheral blood" @default.
- W2069953162 cites W137283645 @default.
- W2069953162 cites W1501050051 @default.
- W2069953162 cites W1505037861 @default.
- W2069953162 cites W1531372132 @default.
- W2069953162 cites W1552193435 @default.
- W2069953162 cites W161630099 @default.
- W2069953162 cites W1748734394 @default.
- W2069953162 cites W1771096450 @default.
- W2069953162 cites W1864823043 @default.
- W2069953162 cites W1890626499 @default.
- W2069953162 cites W1933088330 @default.
- W2069953162 cites W1963610644 @default.
- W2069953162 cites W1966371639 @default.
- W2069953162 cites W1966623655 @default.
- W2069953162 cites W1969245473 @default.
- W2069953162 cites W2012485638 @default.
- W2069953162 cites W2031370899 @default.
- W2069953162 cites W2033792142 @default.
- W2069953162 cites W2041524945 @default.
- W2069953162 cites W2055070696 @default.
- W2069953162 cites W2060333964 @default.
- W2069953162 cites W2071034864 @default.
- W2069953162 cites W2103117450 @default.
- W2069953162 cites W2103606399 @default.
- W2069953162 cites W2105514165 @default.
- W2069953162 cites W2110067379 @default.
- W2069953162 cites W2116112925 @default.
- W2069953162 cites W2120682913 @default.
- W2069953162 cites W2132864109 @default.
- W2069953162 cites W2142191611 @default.
- W2069953162 cites W2144966307 @default.
- W2069953162 cites W2150211095 @default.
- W2069953162 cites W2150697333 @default.
- W2069953162 cites W2159352345 @default.
- W2069953162 cites W2162992800 @default.
- W2069953162 cites W2163379768 @default.
- W2069953162 cites W2165919275 @default.
- W2069953162 cites W2185879634 @default.
- W2069953162 cites W2218220018 @default.
- W2069953162 cites W2316461382 @default.
- W2069953162 cites W2324339443 @default.
- W2069953162 cites W2408853845 @default.
- W2069953162 cites W2409127913 @default.
- W2069953162 cites W2412426396 @default.
- W2069953162 cites W2436610849 @default.
- W2069953162 cites W3112391997 @default.
- W2069953162 doi "https://doi.org/10.1038/bjc.1997.331" @default.
- W2069953162 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2223791" @default.
- W2069953162 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9218728" @default.
- W2069953162 hasPublicationYear "1997" @default.
- W2069953162 type Work @default.
- W2069953162 sameAs 2069953162 @default.
- W2069953162 citedByCount "26" @default.
- W2069953162 crossrefType "journal-article" @default.
- W2069953162 hasAuthorship W2069953162A5016904876 @default.
- W2069953162 hasAuthorship W2069953162A5019749108 @default.
- W2069953162 hasAuthorship W2069953162A5044329653 @default.
- W2069953162 hasAuthorship W2069953162A5049603142 @default.
- W2069953162 hasAuthorship W2069953162A5051348515 @default.
- W2069953162 hasAuthorship W2069953162A5063113967 @default.
- W2069953162 hasAuthorship W2069953162A5091383507 @default.
- W2069953162 hasBestOaLocation W20699531621 @default.
- W2069953162 hasConcept C104317684 @default.
- W2069953162 hasConcept C105580179 @default.
- W2069953162 hasConcept C121608353 @default.
- W2069953162 hasConcept C142724271 @default.
- W2069953162 hasConcept C147483822 @default.
- W2069953162 hasConcept C153911025 @default.
- W2069953162 hasConcept C156719811 @default.
- W2069953162 hasConcept C203014093 @default.
- W2069953162 hasConcept C2777546739 @default.
- W2069953162 hasConcept C2779013556 @default.
- W2069953162 hasConcept C2780007613 @default.
- W2069953162 hasConcept C35866371 @default.
- W2069953162 hasConcept C40767141 @default.
- W2069953162 hasConcept C4692481 @default.
- W2069953162 hasConcept C48023723 @default.
- W2069953162 hasConcept C49105822 @default.
- W2069953162 hasConcept C54355233 @default.
- W2069953162 hasConcept C55493867 @default.
- W2069953162 hasConcept C71924100 @default.
- W2069953162 hasConcept C86803240 @default.
- W2069953162 hasConceptScore W2069953162C104317684 @default.
- W2069953162 hasConceptScore W2069953162C105580179 @default.
- W2069953162 hasConceptScore W2069953162C121608353 @default.
- W2069953162 hasConceptScore W2069953162C142724271 @default.