Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069965896> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2069965896 endingPage "286" @default.
- W2069965896 startingPage "279" @default.
- W2069965896 abstract "A recently introduced thermostable RNase H was tested to determine its effectiveness in RNase H mapping reactions. Procedures are described which should have general use with both the thermostable and theEscherichia coliRNase H enzymes. Using the thermostable RNase H at higher temperatures extends the range of oligodeoxyribonucleotide/RNA combinations that yield satisfactory results. Northern blot analyses of total RNA was used to demonstrate that native RNAs can be analyzed by oligodeoxyribonucleotide directed RNase H digestion with minimal sample processing as long as care is taken to maintain thermal stringency both during reaction assembly and termination. Increased thermal stringency allows for higher DNA concentrations to ensure complete site-specific digestion of target RNAs or to permit simultaneous cleavage with multiple oligodeoxyribonucleotides. Partial digests can also be controlled by manipulating oligodeoxyribonucleotide concentrations. In addition, the thermostable RNase H was shown to be active at magnesium ion concentrations as low as 0.1 mm. This allows for optimization of Mg2+effects on overall sample integrity and DNA/RNA interactions over at least a 20-fold range (2.0–0.1 mm)." @default.
- W2069965896 created "2016-06-24" @default.
- W2069965896 creator A5008684471 @default.
- W2069965896 creator A5059385549 @default.
- W2069965896 date "1997-05-01" @default.
- W2069965896 modified "2023-09-27" @default.
- W2069965896 title "Use of Thermostable andEscherichia coliRNase H in RNA Mapping Studies" @default.
- W2069965896 cites W1484728386 @default.
- W2069965896 cites W1551358827 @default.
- W2069965896 cites W1572721033 @default.
- W2069965896 cites W1969818257 @default.
- W2069965896 cites W1974450466 @default.
- W2069965896 cites W1975686151 @default.
- W2069965896 cites W1994860933 @default.
- W2069965896 cites W2002944191 @default.
- W2069965896 cites W2011966814 @default.
- W2069965896 cites W2018025598 @default.
- W2069965896 cites W2045202900 @default.
- W2069965896 cites W2046884078 @default.
- W2069965896 cites W2048544201 @default.
- W2069965896 cites W2080543533 @default.
- W2069965896 cites W2085405727 @default.
- W2069965896 cites W4243005281 @default.
- W2069965896 cites W4294216491 @default.
- W2069965896 doi "https://doi.org/10.1006/abio.1997.2059" @default.
- W2069965896 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9177689" @default.
- W2069965896 hasPublicationYear "1997" @default.
- W2069965896 type Work @default.
- W2069965896 sameAs 2069965896 @default.
- W2069965896 citedByCount "11" @default.
- W2069965896 countsByYear W20699658962016 @default.
- W2069965896 crossrefType "journal-article" @default.
- W2069965896 hasAuthorship W2069965896A5008684471 @default.
- W2069965896 hasAuthorship W2069965896A5059385549 @default.
- W2069965896 hasConcept C104317684 @default.
- W2069965896 hasConcept C10879258 @default.
- W2069965896 hasConcept C151730666 @default.
- W2069965896 hasConcept C153911025 @default.
- W2069965896 hasConcept C175156509 @default.
- W2069965896 hasConcept C181199279 @default.
- W2069965896 hasConcept C185592680 @default.
- W2069965896 hasConcept C24107716 @default.
- W2069965896 hasConcept C43369102 @default.
- W2069965896 hasConcept C552990157 @default.
- W2069965896 hasConcept C55493867 @default.
- W2069965896 hasConcept C67705224 @default.
- W2069965896 hasConcept C69766542 @default.
- W2069965896 hasConcept C86803240 @default.
- W2069965896 hasConceptScore W2069965896C104317684 @default.
- W2069965896 hasConceptScore W2069965896C10879258 @default.
- W2069965896 hasConceptScore W2069965896C151730666 @default.
- W2069965896 hasConceptScore W2069965896C153911025 @default.
- W2069965896 hasConceptScore W2069965896C175156509 @default.
- W2069965896 hasConceptScore W2069965896C181199279 @default.
- W2069965896 hasConceptScore W2069965896C185592680 @default.
- W2069965896 hasConceptScore W2069965896C24107716 @default.
- W2069965896 hasConceptScore W2069965896C43369102 @default.
- W2069965896 hasConceptScore W2069965896C552990157 @default.
- W2069965896 hasConceptScore W2069965896C55493867 @default.
- W2069965896 hasConceptScore W2069965896C67705224 @default.
- W2069965896 hasConceptScore W2069965896C69766542 @default.
- W2069965896 hasConceptScore W2069965896C86803240 @default.
- W2069965896 hasIssue "2" @default.
- W2069965896 hasLocation W20699658961 @default.
- W2069965896 hasLocation W20699658962 @default.
- W2069965896 hasOpenAccess W2069965896 @default.
- W2069965896 hasPrimaryLocation W20699658961 @default.
- W2069965896 hasRelatedWork W1906502852 @default.
- W2069965896 hasRelatedWork W1963992374 @default.
- W2069965896 hasRelatedWork W1985875854 @default.
- W2069965896 hasRelatedWork W2022965478 @default.
- W2069965896 hasRelatedWork W2055618423 @default.
- W2069965896 hasRelatedWork W2080459625 @default.
- W2069965896 hasRelatedWork W2085300355 @default.
- W2069965896 hasRelatedWork W2114301480 @default.
- W2069965896 hasRelatedWork W2433397146 @default.
- W2069965896 hasRelatedWork W2528056874 @default.
- W2069965896 hasVolume "247" @default.
- W2069965896 isParatext "false" @default.
- W2069965896 isRetracted "false" @default.
- W2069965896 magId "2069965896" @default.
- W2069965896 workType "article" @default.