Matches in SemOpenAlex for { <https://semopenalex.org/work/W2069968651> ?p ?o ?g. }
- W2069968651 endingPage "948" @default.
- W2069968651 startingPage "937" @default.
- W2069968651 abstract "Deacetoxycephalosporin C synthase (DAOCS) is an iron(II) and 2-oxoglutarate-dependent oxygenase that catalyzes the conversion of penicillin N to deacetoxycephalosporin C, the committed step in the biosynthesis of cephalosporin antibiotics. The crystal structure of DAOCS revealed that the C terminus of one molecule is inserted into the active site of its neighbor in a cyclical fashion within a trimeric unit. This arrangement has hindered the generation of crystalline enzyme-substrate complexes. Therefore, we constructed a series of DAOCS mutants with modified C termini. Oxidation of 2-oxoglutarate was significantly uncoupled from oxidation of the penicillin substrate in certain truncated mutants. The extent of uncoupling varied with the number of residues deleted and the penicillin substrate used. Crystal structures were determined for the DeltaR306 mutant complexed with iron(II) and 2-oxoglutarate (to 2.10 A) and the DeltaR306A mutant complexed with iron(II), succinate and unhydrated carbon dioxide (to 1.96 A). The latter may mimic a product complex, and supports proposals for a metal-bound CO(2) intermediate during catalysis." @default.
- W2069968651 created "2016-06-24" @default.
- W2069968651 creator A5025673952 @default.
- W2069968651 creator A5036024913 @default.
- W2069968651 creator A5036172001 @default.
- W2069968651 creator A5042398293 @default.
- W2069968651 creator A5059436482 @default.
- W2069968651 creator A5088578643 @default.
- W2069968651 date "2001-05-01" @default.
- W2069968651 modified "2023-10-17" @default.
- W2069968651 title "Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS)" @default.
- W2069968651 cites W1514604371 @default.
- W2069968651 cites W1539796472 @default.
- W2069968651 cites W1586792701 @default.
- W2069968651 cites W1591680814 @default.
- W2069968651 cites W1597350167 @default.
- W2069968651 cites W1650854146 @default.
- W2069968651 cites W1972878464 @default.
- W2069968651 cites W1980467950 @default.
- W2069968651 cites W1987763871 @default.
- W2069968651 cites W1988073167 @default.
- W2069968651 cites W1989298691 @default.
- W2069968651 cites W1991897387 @default.
- W2069968651 cites W1992802317 @default.
- W2069968651 cites W1994270304 @default.
- W2069968651 cites W1995017064 @default.
- W2069968651 cites W2001641653 @default.
- W2069968651 cites W2007151289 @default.
- W2069968651 cites W2007243697 @default.
- W2069968651 cites W2008311769 @default.
- W2069968651 cites W2009934503 @default.
- W2069968651 cites W2013083986 @default.
- W2069968651 cites W2015304573 @default.
- W2069968651 cites W2020108475 @default.
- W2069968651 cites W2022277606 @default.
- W2069968651 cites W2036931150 @default.
- W2069968651 cites W2041796853 @default.
- W2069968651 cites W2044917996 @default.
- W2069968651 cites W2056505663 @default.
- W2069968651 cites W2057222541 @default.
- W2069968651 cites W2069233285 @default.
- W2069968651 cites W2071780808 @default.
- W2069968651 cites W2076694379 @default.
- W2069968651 cites W2080528351 @default.
- W2069968651 cites W2083480863 @default.
- W2069968651 cites W2085815993 @default.
- W2069968651 cites W2095788149 @default.
- W2069968651 cites W2111973306 @default.
- W2069968651 cites W2116275717 @default.
- W2069968651 cites W2118291488 @default.
- W2069968651 cites W2119773701 @default.
- W2069968651 cites W2121849466 @default.
- W2069968651 cites W2338151994 @default.
- W2069968651 cites W2409565639 @default.
- W2069968651 cites W2888511473 @default.
- W2069968651 cites W40527201 @default.
- W2069968651 doi "https://doi.org/10.1006/jmbi.2001.4649" @default.
- W2069968651 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11352583" @default.
- W2069968651 hasPublicationYear "2001" @default.
- W2069968651 type Work @default.
- W2069968651 sameAs 2069968651 @default.
- W2069968651 citedByCount "94" @default.
- W2069968651 countsByYear W20699686512012 @default.
- W2069968651 countsByYear W20699686512013 @default.
- W2069968651 countsByYear W20699686512014 @default.
- W2069968651 countsByYear W20699686512015 @default.
- W2069968651 countsByYear W20699686512016 @default.
- W2069968651 countsByYear W20699686512017 @default.
- W2069968651 countsByYear W20699686512018 @default.
- W2069968651 countsByYear W20699686512019 @default.
- W2069968651 countsByYear W20699686512020 @default.
- W2069968651 countsByYear W20699686512021 @default.
- W2069968651 countsByYear W20699686512022 @default.
- W2069968651 countsByYear W20699686512023 @default.
- W2069968651 crossrefType "journal-article" @default.
- W2069968651 hasAuthorship W2069968651A5025673952 @default.
- W2069968651 hasAuthorship W2069968651A5036024913 @default.
- W2069968651 hasAuthorship W2069968651A5036172001 @default.
- W2069968651 hasAuthorship W2069968651A5042398293 @default.
- W2069968651 hasAuthorship W2069968651A5059436482 @default.
- W2069968651 hasAuthorship W2069968651A5088578643 @default.
- W2069968651 hasConcept C104317684 @default.
- W2069968651 hasConcept C115624301 @default.
- W2069968651 hasConcept C134121241 @default.
- W2069968651 hasConcept C143065580 @default.
- W2069968651 hasConcept C178790620 @default.
- W2069968651 hasConcept C181199279 @default.
- W2069968651 hasConcept C185592680 @default.
- W2069968651 hasConcept C18903297 @default.
- W2069968651 hasConcept C191897082 @default.
- W2069968651 hasConcept C192562407 @default.
- W2069968651 hasConcept C2776067932 @default.
- W2069968651 hasConcept C2777289219 @default.
- W2069968651 hasConcept C32909587 @default.
- W2069968651 hasConcept C41183919 @default.
- W2069968651 hasConcept C501593827 @default.
- W2069968651 hasConcept C553450214 @default.
- W2069968651 hasConcept C55493867 @default.