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- W2069992478 abstract "Mengo virus was cultivated <i>in vitro </i>in Ehrlich ascites carcinoma (EAC) cell suspensions. After infection with well-adapted viruses (50 passages), using a MOI of 20 TCD<sub>50</sub>/ cell, the replication cycle showed the following course: slow begin of virus multiplication and virus-induced RNA synthesis on the 4th h, the maximum of virus yield (titre of infectious virus) was obtained on the 12th h after washing out the unadsorbed viruses; the maximum of viral RNA synthesis was determined between the 7th and 8th h (measured as incorporation of <sup>3</sup>H-uridine into acid-insoluble material of infected and actinomycin D-treated cultures), and the decrease lasted up to 12 h. The increase of cell-free viruses within the medium began on the 8th h and showed the highest value on the 14th h. Mengo virus grown in EAC cells exhibited demonstrable haemagglutinin titres." @default.
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- W2069992478 date "1974-01-01" @default.
- W2069992478 modified "2023-09-25" @default.
- W2069992478 title "Vermehrung von Mengovirus in Suspensionskulturen von Ehrlich-Aszites-Karzinom-Zellen" @default.
- W2069992478 doi "https://doi.org/10.1159/000162567" @default.
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