FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2070036593> ?p ?o ?g. }

• W2070036593 endingPage "1098" @default.
• W2070036593 startingPage "1089" @default.
• W2070036593 abstract "FSH bioactivity was measured by means of FSH-dependent aromatase activity (conversion of androgen substrate to estradiol). Assay sensitivity was optimized by the use of immature (7-10 days old) rats as Sertoli cell donors, serum-free medium for incubation, phosphodiesterase inhibitor (methylisobutylxanthinine), serial dilution of FSH in medium containing 1% BSA, delayed addition of FSH for 72 h after cell plating, and 19-hydroxyandrostenedione (2.5 X 10(-6) M) as the aromatizable androgen substrate. The method consisted of subjecting the decapsulated immature rat testes to a 2-step collagenase dispersion, plating the cells in medium [Dulbecco's Modified Eagle's Medium-Ham's F-10 (1:1)] containing growth factors and methylisobutylxanthinine for 72 h, adding increasing doses of FSH to the standard curve or small volumes of serum to the test vials as well as 19-hydroxyandrostenedione for 24 h, and measuring estradiol by RIA in dilutions of the medium. Using NIAMDD human (h) FSH-2 as the bioassay standard, the useful range of the assay was 0.01-5.0 ng/ml. Specificity was determined by the addition of graded doses of hLH, hTSH, ACTH, hGH, hPRL, and hCG. The minor degree of FSH bioactivity observed in a few hormone preparations was accounted for by the degree of FSH contamination in them. Mean intra- and interassay coefficients of variation were 9% and 11%, and the index of precision was 0.049. This bioassay was used to determine the bioactive FSH content of pituitary extracts, tissue culture media, elutions from columns, and isoelectrically focused samples. More importantly, small quantities of human sera gave responses parallel to the standard curve in a minimum of two dilutions. The bio- to immunoreactive ratios, expressed as the mean +/- SEM (NIAMDD-hFSH-2), were 0.66 +/- 0.2 in boys (n = 6), 0.78 +/- 0.2 in pubertal girls (n = 6), 1.18 +/- 0.2 in men (n = 13), 1.24 +/- 0.1 in postmenopausal women (n = 30), 1.94 +/- 0.3 in the follicular phase (n = 19), 6.2 +/- 1.4 in the ovulatory phase (n = 19), and 1.6 +/- 0.4 in the luteal phase (n = 19) of the normal menstrual cycle. These results indicate that the bio- to immunoreactive hFSH ratio in the circulation, is dependent upon the hormonal milieu of the subject." @default.
• W2070036593 created "2016-06-24" @default.
• W2070036593 creator A5029119143 @default.
• W2070036593 creator A5030252141 @default.
• W2070036593 creator A5079895201 @default.
• W2070036593 date "1987-09-01" @default.
• W2070036593 modified "2023-10-06" @default.
• W2070036593 title "An Improved in Vitro Bioassay for Follicle-Stimulating Hormone (FSH): Suitable for Measurement of FSH in Unextracted Human Serum*" @default.
• W2070036593 cites W1504821571 @default.
• W2070036593 cites W1510260956 @default.
• W2070036593 cites W1545354040 @default.
• W2070036593 cites W1548637624 @default.
• W2070036593 cites W1569589672 @default.
• W2070036593 cites W196452131 @default.
• W2070036593 cites W1966418425 @default.
• W2070036593 cites W1968978596 @default.
• W2070036593 cites W1971601768 @default.
• W2070036593 cites W1973151872 @default.
• W2070036593 cites W1975306695 @default.
• W2070036593 cites W1976138505 @default.
• W2070036593 cites W1987916591 @default.
• W2070036593 cites W1988366964 @default.
• W2070036593 cites W1989129619 @default.
• W2070036593 cites W1990280547 @default.
• W2070036593 cites W1999208899 @default.
• W2070036593 cites W2005446108 @default.
• W2070036593 cites W2007285655 @default.
• W2070036593 cites W2007447681 @default.
• W2070036593 cites W2010220920 @default.
• W2070036593 cites W2013796456 @default.
• W2070036593 cites W2016546602 @default.
• W2070036593 cites W2026790499 @default.
• W2070036593 cites W2028528771 @default.
• W2070036593 cites W2030759588 @default.
• W2070036593 cites W2030848234 @default.
• W2070036593 cites W2032280488 @default.
• W2070036593 cites W2041710171 @default.
• W2070036593 cites W2044897122 @default.
• W2070036593 cites W2065637222 @default.
• W2070036593 cites W2069729167 @default.
• W2070036593 cites W2074343140 @default.
• W2070036593 cites W2074682982 @default.
• W2070036593 cites W2077773750 @default.
• W2070036593 cites W2079563753 @default.
• W2070036593 cites W2081343590 @default.
• W2070036593 cites W2085837932 @default.
• W2070036593 cites W2087553110 @default.
• W2070036593 cites W2088931910 @default.
• W2070036593 cites W2090497361 @default.
• W2070036593 cites W2094381759 @default.
• W2070036593 cites W2099724663 @default.
• W2070036593 cites W2100100394 @default.
• W2070036593 cites W2116805862 @default.
• W2070036593 cites W2149936339 @default.
• W2070036593 cites W2154638287 @default.
• W2070036593 cites W2159278424 @default.
• W2070036593 cites W2159844624 @default.
• W2070036593 cites W2410466883 @default.
• W2070036593 cites W2412503111 @default.
• W2070036593 cites W2443967682 @default.
• W2070036593 doi "https://doi.org/10.1210/endo-121-3-1089" @default.
• W2070036593 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3113917" @default.
• W2070036593 hasPublicationYear "1987" @default.
• W2070036593 type Work @default.
• W2070036593 sameAs 2070036593 @default.
• W2070036593 citedByCount "84" @default.
• W2070036593 countsByYear W20700365932012 @default.
• W2070036593 countsByYear W20700365932013 @default.
• W2070036593 countsByYear W20700365932015 @default.
• W2070036593 countsByYear W20700365932016 @default.
• W2070036593 countsByYear W20700365932019 @default.
• W2070036593 crossrefType "journal-article" @default.
• W2070036593 hasAuthorship W2070036593A5029119143 @default.
• W2070036593 hasAuthorship W2070036593A5030252141 @default.
• W2070036593 hasAuthorship W2070036593A5079895201 @default.
• W2070036593 hasConcept C104488531 @default.
• W2070036593 hasConcept C126322002 @default.
• W2070036593 hasConcept C134018914 @default.
• W2070036593 hasConcept C142724271 @default.
• W2070036593 hasConcept C176685330 @default.
• W2070036593 hasConcept C181199279 @default.
• W2070036593 hasConcept C184112429 @default.
• W2070036593 hasConcept C185592680 @default.
• W2070036593 hasConcept C204787440 @default.
• W2070036593 hasConcept C2778394429 @default.
• W2070036593 hasConcept C2778575703 @default.
• W2070036593 hasConcept C2778656385 @default.
• W2070036593 hasConcept C2779279165 @default.
• W2070036593 hasConcept C54355233 @default.
• W2070036593 hasConcept C55493867 @default.
• W2070036593 hasConcept C71315377 @default.
• W2070036593 hasConcept C71924100 @default.
• W2070036593 hasConcept C86803240 @default.
• W2070036593 hasConceptScore W2070036593C104488531 @default.
• W2070036593 hasConceptScore W2070036593C126322002 @default.
• W2070036593 hasConceptScore W2070036593C134018914 @default.
• W2070036593 hasConceptScore W2070036593C142724271 @default.
• W2070036593 hasConceptScore W2070036593C176685330 @default.

• next