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- W2070092912 abstract "Grapevines infected with Tomato ring spot virus (ToRSV) pose an economic risk for growers in the northeastern United States. This study describes a one-step real-time reverse-transcription polymerase chain reaction (RT-PCR) SYBR Green assay for detecting ToRSV in grapevines. Two newly designed primer pairs based on the ToRSV coat protein gene sequence were evaluated for specificity and optimized for a SYBR Green assay. The primer pair ToRSV1f/1r yielded a 130-bp product with strong primer-dimer products, whereas the primer pair ToRSV2f/2r yielded a 330-bp product with weak primer dimer products. Real-time RT-PCR detected ToRSV in more naturally infected grapevines maintained in the greenhouse than did enzyme-linked immunosorbent assay. The nucleotide sequences of the fragments amplified from grapevine growing in Pennsylvania using real-time PCR were divergent from previously published sequences." @default.
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- W2070092912 date "2007-09-01" @default.
- W2070092912 modified "2023-09-28" @default.
- W2070092912 title "Development of a Real-Time RT-PCR SYBR Green Assay for<i>Tomato ring spot virus</i>in Grape" @default.
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- W2070092912 doi "https://doi.org/10.1094/pdis-91-9-1083" @default.
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