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- W2070110890 abstract "Enzyme-modified microelectrodes were developed for the indirect amperometric detection of acetylcholine and choline following separation by capillary electrophoresis. Electrodes were prepared by first sequentially electrodepositing polypyrrole and polytyramine from the monomers on a 200-μm platinum electrode. The polymer bilayer provides enhanced selectivity and pendant amino groups for covalent coupling of acetylcholinesterase (AChE) and choline oxidase (ChO) by their reaction with glutaraldehyde. The AChE/ChO-modified electrode was then employed as an end-column detector to determine acetylcholine and choline with and without the internal standard butyrylcholine. Excellent operational stability during 2 days of continuous use was observed, with detection limits of 2 μM or 50 fmol for both acetylcholine and choline. The response from potential interferences, such as dopamine, catechol, and norepinephrine, were significantly reduced in comparison to a bare platinum electrode. The utility of this approach was demonstrated by monitoring the uptake of choline into synaptosomes." @default.
- W2070110890 created "2016-06-24" @default.
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- W2070110890 date "2002-09-07" @default.
- W2070110890 modified "2023-09-23" @default.
- W2070110890 title "Enhanced Measurement Stability and Selectivity for Choline and Acetylcholine by Capillary Electrophoresis with Electrochemical Detection at a Covalently Linked Enzyme-Modified Electrode" @default.
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- W2070110890 doi "https://doi.org/10.1021/ac020286l" @default.
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