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• W2070167188 endingPage "327" @default.
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• W2070167188 abstract "In vitro cell biocompatibility models are crucial in the study of any newly synthesized material. Our focus has been on the development of a new class of biocompatible, degradable, high-strength polymeric materials, the poly(anhydride-co-imides), for use in bone regeneration. This study examined osteoblast cell adherence, proliferation, viability, and phenotypic preservation on the surface of the poly(anhydride-co-imide) poly[pyromellitylimidoalanine (PMA-ala):1,6-bis(carboxyphenoxy) hexane (CPH)] over a period of time. Cell proliferation on PMA-ala:CPH degradable matrices over 21 days was examined. Throughout the 21-day period of study, osteoblast proliferation was similar on PMA-ala:CPH and on tissue culture polystyrene controls. Osteoblasts maintained their characteristic morphology as demonstrated by both scanning electron microscopy and immunofluorescence studies. Alkaline phosphatase activity for cells grown on PMA-ala:CPH was confirmed. Retention of the osteoblastic phenotype was demonstrated using immunofluorescence techniques and staining with antibodies against osteocalcin (an extracellular matrix protein of bone) and osteopontin (a marker of cell adhesion). Radioimmunoassay results provided evidence that levels of osteocalcin production by osteoblasts were similar when cells were cultured on PMA-ala:CPH and on tissue culture polystyrene controls. The present study provided evidence of normal osteoblast function on PMA-ala:CPH surfaces. PMA-ala:CPH may therefore be useful as a synthetic material for orthopedic applications. © 1999 John Wiley & Sons, Inc. J Biomed Mater Res (Appl Biomater) 48: 322–327, 1999" @default.
• W2070167188 created "2016-06-24" @default.
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• W2070167188 date "1999-01-01" @default.
• W2070167188 modified "2023-10-10" @default.
• W2070167188 title "Proliferation, morphology, and protein expression by osteoblasts cultured on poly(anhydride-co-imides)" @default.
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• W2070167188 doi "https://doi.org/10.1002/(sici)1097-4636(1999)48:3<322::aid-jbm17>3.0.co;2-u" @default.
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