FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2070180947> ?p ?o ?g. }

• W2070180947 endingPage "2525" @default.
• W2070180947 startingPage "2517" @default.
• W2070180947 abstract "Ebola virus assembles and buds from the inner leaflet of the plasma membrane of mammalian cells, which is primarily attributed to its major matrix protein VP40. Oligomerization of VP40 has been shown to be essential to the life cycle of the virus including formation of virions from infected cells. To date, VP40 oligomerization has mainly been assessed by chemical cross-linking following cell fractionation studies with VP40 transfected cells. This has made it difficult to discern the spatial and temporal dynamics of VP40 oligomerization. To gain a better understanding of the VP40 assembly and oligomerization process in live cells, we have employed real-time imaging of enhanced green fluorescent protein tagged VP40. Here, we use both confocal and total internal reflection microscopy coupled with number and brightness analysis to show that VP40 oligomers are localized on the plasma membrane and are highly enriched at sites of membrane protrusion, consistent with sites of viral budding. These filamentous plasma membrane protrusion sites harbor VP40 hexamers, octamers, and higher order oligomers. Consistent with previous reports, abrogation of VP40 oligomerization through mutagenesis greatly diminished VP40 egress and also abolished membrane protrusion sites enriched with VP40. In sum, real-time single-molecule imaging of fluorescently labeled Ebola VP40 is able to resolve the spatial and temporal dynamics of VP40 oligomerization." @default.
• W2070180947 created "2016-06-24" @default.
• W2070180947 creator A5005039590 @default.
• W2070180947 creator A5020924158 @default.
• W2070180947 creator A5022670010 @default.
• W2070180947 creator A5078195240 @default.
• W2070180947 date "2012-06-01" @default.
• W2070180947 modified "2023-09-27" @default.
• W2070180947 title "Investigation of Ebola VP40 Assembly and Oligomerization in Live Cells Using Number and Brightness Analysis" @default.
• W2070180947 cites W1977240303 @default.
• W2070180947 cites W1982859702 @default.
• W2070180947 cites W1991131490 @default.
• W2070180947 cites W1998641585 @default.
• W2070180947 cites W2016189813 @default.
• W2070180947 cites W2016838605 @default.
• W2070180947 cites W2018741273 @default.
• W2070180947 cites W2018869629 @default.
• W2070180947 cites W2022595858 @default.
• W2070180947 cites W2022719897 @default.
• W2070180947 cites W2023670906 @default.
• W2070180947 cites W2024375090 @default.
• W2070180947 cites W2029169661 @default.
• W2070180947 cites W2041902881 @default.
• W2070180947 cites W2042182707 @default.
• W2070180947 cites W2053240860 @default.
• W2070180947 cites W2068761461 @default.
• W2070180947 cites W2069796283 @default.
• W2070180947 cites W2071607445 @default.
• W2070180947 cites W2071967966 @default.
• W2070180947 cites W2073857392 @default.
• W2070180947 cites W2074400765 @default.
• W2070180947 cites W2077173334 @default.
• W2070180947 cites W2078079353 @default.
• W2070180947 cites W2081877186 @default.
• W2070180947 cites W2093145534 @default.
• W2070180947 cites W2094644220 @default.
• W2070180947 cites W2096284021 @default.
• W2070180947 cites W2111014305 @default.
• W2070180947 cites W2112956516 @default.
• W2070180947 cites W2116416473 @default.
• W2070180947 cites W2121015322 @default.
• W2070180947 cites W2126069516 @default.
• W2070180947 cites W2127806688 @default.
• W2070180947 cites W2131758244 @default.
• W2070180947 cites W2148915854 @default.
• W2070180947 cites W2149669194 @default.
• W2070180947 cites W2156633263 @default.
• W2070180947 cites W2160796054 @default.
• W2070180947 cites W2165381688 @default.
• W2070180947 cites W2171502794 @default.
• W2070180947 cites W2171864254 @default.
• W2070180947 cites W339361884 @default.
• W2070180947 cites W4211044441 @default.
• W2070180947 doi "https://doi.org/10.1016/j.bpj.2012.04.022" @default.
• W2070180947 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3368128" @default.
• W2070180947 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22713567" @default.
• W2070180947 hasPublicationYear "2012" @default.
• W2070180947 type Work @default.
• W2070180947 sameAs 2070180947 @default.
• W2070180947 citedByCount "69" @default.
• W2070180947 countsByYear W20701809472012 @default.
• W2070180947 countsByYear W20701809472013 @default.
• W2070180947 countsByYear W20701809472014 @default.
• W2070180947 countsByYear W20701809472015 @default.
• W2070180947 countsByYear W20701809472016 @default.
• W2070180947 countsByYear W20701809472017 @default.
• W2070180947 countsByYear W20701809472018 @default.
• W2070180947 countsByYear W20701809472019 @default.
• W2070180947 countsByYear W20701809472020 @default.
• W2070180947 countsByYear W20701809472021 @default.
• W2070180947 countsByYear W20701809472022 @default.
• W2070180947 countsByYear W20701809472023 @default.
• W2070180947 crossrefType "journal-article" @default.
• W2070180947 hasAuthorship W2070180947A5005039590 @default.
• W2070180947 hasAuthorship W2070180947A5020924158 @default.
• W2070180947 hasAuthorship W2070180947A5022670010 @default.
• W2070180947 hasAuthorship W2070180947A5078195240 @default.
• W2070180947 hasBestOaLocation W20701809471 @default.
• W2070180947 hasConcept C12554922 @default.
• W2070180947 hasConcept C159047783 @default.
• W2070180947 hasConcept C185592680 @default.
• W2070180947 hasConcept C2522874641 @default.
• W2070180947 hasConcept C2777469322 @default.
• W2070180947 hasConcept C68434959 @default.
• W2070180947 hasConcept C86295872 @default.
• W2070180947 hasConcept C86803240 @default.
• W2070180947 hasConcept C95444343 @default.
• W2070180947 hasConceptScore W2070180947C12554922 @default.
• W2070180947 hasConceptScore W2070180947C159047783 @default.
• W2070180947 hasConceptScore W2070180947C185592680 @default.
• W2070180947 hasConceptScore W2070180947C2522874641 @default.
• W2070180947 hasConceptScore W2070180947C2777469322 @default.
• W2070180947 hasConceptScore W2070180947C68434959 @default.
• W2070180947 hasConceptScore W2070180947C86295872 @default.
• W2070180947 hasConceptScore W2070180947C86803240 @default.
• W2070180947 hasConceptScore W2070180947C95444343 @default.
• W2070180947 hasIssue "11" @default.
• W2070180947 hasLocation W20701809471 @default.

• next