FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2070214298> ?p ?o ?g. }

• W2070214298 endingPage "329" @default.
• W2070214298 startingPage "322" @default.
• W2070214298 abstract "Brucella abortus is a Gram-negative, facultative intracellular bacterial pathogen of human and other animals. Brucella lipopolysaccharide has been identified as an important virulence factor. In this study, the ABC transporter ATPase gene (BAB1_0542) of B. abortus strain S2308 was inactivated by deleting a 446-bp fragment from the gene, thereby generating the mutant strain, S2308ΔATP. Real time PCR analysis confirmed the inactivation of this gene with no polar effect on the transcription of adjacent genes on the chromosome. The mutant was identified as a rough phenotype strain using heat agglutination test and crystal violet staining. The mutant strain had a different growth rate in Tryptic Soy Broth (TSB), compared to the wild type S2308 strain. Moreover, the mutant strain showed attenuated virulence in vitro and in vivo in RAW264.7 macrophages and Balb/c mice, respectively. Complementation of the mutant strain recovered the smooth phenotype of the bacteria and the complemented strain C2308ΔATP survived for more than four weeks in Balb/c mice, comparable to wild type strain S2308. Furthermore, immunization with the mutant strain protected mice from virulent strain challenge, which suggests the potential for the mutant strain S2308ΔATP as a future vaccine candidate. MHC I, MHC II and co-stimulatory molecule expression levels in mice following infection of S2308ΔATP and S2308 were also investigated." @default.
• W2070214298 created "2016-06-24" @default.
• W2070214298 creator A5007024868 @default.
• W2070214298 creator A5015002344 @default.
• W2070214298 creator A5036883240 @default.
• W2070214298 creator A5045037679 @default.
• W2070214298 creator A5053204257 @default.
• W2070214298 creator A5065570155 @default.
• W2070214298 creator A5071115916 @default.
• W2070214298 creator A5074633497 @default.
• W2070214298 creator A5082474774 @default.
• W2070214298 date "2013-06-01" @default.
• W2070214298 modified "2023-10-03" @default.
• W2070214298 title "Inactivation of the ABC transporter ATPase gene in Brucella abortus strain 2308 attenuated the virulence of the bacteria" @default.
• W2070214298 cites W102233043 @default.
• W2070214298 cites W114420991 @default.
• W2070214298 cites W1563292811 @default.
• W2070214298 cites W1601058961 @default.
• W2070214298 cites W1898933784 @default.
• W2070214298 cites W1903844680 @default.
• W2070214298 cites W1972858806 @default.
• W2070214298 cites W1973748344 @default.
• W2070214298 cites W1978417284 @default.
• W2070214298 cites W1988558949 @default.
• W2070214298 cites W1996929743 @default.
• W2070214298 cites W1998602982 @default.
• W2070214298 cites W2010346758 @default.
• W2070214298 cites W2023162414 @default.
• W2070214298 cites W2039060736 @default.
• W2070214298 cites W2050589013 @default.
• W2070214298 cites W2052175329 @default.
• W2070214298 cites W2097754561 @default.
• W2070214298 cites W2108712424 @default.
• W2070214298 cites W2122310864 @default.
• W2070214298 cites W2132451297 @default.
• W2070214298 cites W2135937013 @default.
• W2070214298 cites W2144586834 @default.
• W2070214298 cites W2149443985 @default.
• W2070214298 cites W2150500173 @default.
• W2070214298 cites W2159188698 @default.
• W2070214298 cites W2159944158 @default.
• W2070214298 doi "https://doi.org/10.1016/j.vetmic.2013.02.017" @default.
• W2070214298 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23507429" @default.
• W2070214298 hasPublicationYear "2013" @default.
• W2070214298 type Work @default.
• W2070214298 sameAs 2070214298 @default.
• W2070214298 citedByCount "25" @default.
• W2070214298 countsByYear W20702142982013 @default.
• W2070214298 countsByYear W20702142982014 @default.
• W2070214298 countsByYear W20702142982015 @default.
• W2070214298 countsByYear W20702142982016 @default.
• W2070214298 countsByYear W20702142982017 @default.
• W2070214298 countsByYear W20702142982018 @default.
• W2070214298 countsByYear W20702142982019 @default.
• W2070214298 countsByYear W20702142982020 @default.
• W2070214298 countsByYear W20702142982021 @default.
• W2070214298 countsByYear W20702142982023 @default.
• W2070214298 crossrefType "journal-article" @default.
• W2070214298 hasAuthorship W2070214298A5007024868 @default.
• W2070214298 hasAuthorship W2070214298A5015002344 @default.
• W2070214298 hasAuthorship W2070214298A5036883240 @default.
• W2070214298 hasAuthorship W2070214298A5045037679 @default.
• W2070214298 hasAuthorship W2070214298A5053204257 @default.
• W2070214298 hasAuthorship W2070214298A5065570155 @default.
• W2070214298 hasAuthorship W2070214298A5071115916 @default.
• W2070214298 hasAuthorship W2070214298A5074633497 @default.
• W2070214298 hasAuthorship W2070214298A5082474774 @default.
• W2070214298 hasConcept C104317684 @default.
• W2070214298 hasConcept C105702510 @default.
• W2070214298 hasConcept C143065580 @default.
• W2070214298 hasConcept C149011108 @default.
• W2070214298 hasConcept C153911025 @default.
• W2070214298 hasConcept C159047783 @default.
• W2070214298 hasConcept C188082640 @default.
• W2070214298 hasConcept C2777692091 @default.
• W2070214298 hasConcept C2778022156 @default.
• W2070214298 hasConcept C2780767784 @default.
• W2070214298 hasConcept C2781028839 @default.
• W2070214298 hasConcept C2781429187 @default.
• W2070214298 hasConcept C44312359 @default.
• W2070214298 hasConcept C54355233 @default.
• W2070214298 hasConcept C54602769 @default.
• W2070214298 hasConcept C60987743 @default.
• W2070214298 hasConcept C86803240 @default.
• W2070214298 hasConcept C89423630 @default.
• W2070214298 hasConceptScore W2070214298C104317684 @default.
• W2070214298 hasConceptScore W2070214298C105702510 @default.
• W2070214298 hasConceptScore W2070214298C143065580 @default.
• W2070214298 hasConceptScore W2070214298C149011108 @default.
• W2070214298 hasConceptScore W2070214298C153911025 @default.
• W2070214298 hasConceptScore W2070214298C159047783 @default.
• W2070214298 hasConceptScore W2070214298C188082640 @default.
• W2070214298 hasConceptScore W2070214298C2777692091 @default.
• W2070214298 hasConceptScore W2070214298C2778022156 @default.
• W2070214298 hasConceptScore W2070214298C2780767784 @default.
• W2070214298 hasConceptScore W2070214298C2781028839 @default.
• W2070214298 hasConceptScore W2070214298C2781429187 @default.
• W2070214298 hasConceptScore W2070214298C44312359 @default.

• next