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- W2070239760 abstract "We have found that the bacterial transposon Tn 7 can recognize and preferentially insert adjacent to triple-helical nucleic acid structures. Both synthetic intermolecular triplexes, formed through the pairing of a short triplex-forming oligonucleotide on a plasmid DNA, and naturally occurring mirror repeat sequences known to form intramolecular triplexes or H-form DNA are preferential targets for Tn 7 insertion in vitro . This target site selectivity depends upon the recognition of the triplex region by a Tn 7 -encoded ATP-using protein, TnsC, which controls Tn 7 target site selection: the interaction of TnsC with the triplex region results in recruitment and activation of the Tn 7 transposase. Recognition of a nucleic acid structural motif provides both new information into the factors that influence Tn 7 's target site selection and broadens its targeting capabilities." @default.
- W2070239760 created "2016-06-24" @default.
- W2070239760 creator A5005725395 @default.
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- W2070239760 date "2000-03-28" @default.
- W2070239760 modified "2023-09-27" @default.
- W2070239760 title "Recognition of triple-helical DNA structures by transposon Tn <i>7</i>" @default.
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- W2070239760 doi "https://doi.org/10.1073/pnas.080061497" @default.
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