FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2070284483> ?p ?o ?g. }

• W2070284483 endingPage "336" @default.
• W2070284483 startingPage "323" @default.
• W2070284483 abstract "NupG from Escherichia coli is the archetype of a family of nucleoside transporters found in several eubacterial groups and has distant homologues in eukaryotes, including man. To facilitate investigation of its molecular mechanism, we developed methods for expressing an oligohistidine-tagged form of NupG both at high levels (>20% of the inner membrane protein) in E. coli and in Xenopus laevis oocytes. In E. coli recombinant NupG transported purine (adenosine) and pyrimidine (uridine) nucleosides with apparent K(m) values of approximately 20-30 microM and transport was energized primarily by the membrane potential component of the proton motive force. Competition experiments in E. coli and measurements of uptake in oocytes confirmed that NupG was a broad-specificity transporter of purine and pyrimidine nucleosides. Importantly, using high-level expression in E. coli and magic-angle spinning cross-polarization solid-state nuclear magnetic resonance, we have for the first time been able directly to measure the binding of the permeant ([1'-(13)C]uridine) to the protein and to assess its relative mobility within the binding site, under non-energized conditions. Purification of over-expressed NupG to near homogeneity by metal chelate affinity chromatography, with retention of transport function in reconstitution assays, was also achieved. Fourier transform infrared and circular dichroism spectroscopy provided further evidence that the purified protein retained its 3D conformation and was predominantly alpha-helical in nature, consistent with a proposed structure containing 12 transmembrane helices. These findings open the way to elucidating the molecular mechanism of transport in this key family of membrane transporters." @default.
• W2070284483 created "2016-06-24" @default.
• W2070284483 creator A5004045461 @default.
• W2070284483 creator A5009942080 @default.
• W2070284483 creator A5012010132 @default.
• W2070284483 creator A5015381194 @default.
• W2070284483 creator A5023251665 @default.
• W2070284483 creator A5027067788 @default.
• W2070284483 creator A5034487444 @default.
• W2070284483 creator A5037331354 @default.
• W2070284483 creator A5055099992 @default.
• W2070284483 creator A5060865138 @default.
• W2070284483 creator A5076361697 @default.
• W2070284483 creator A5078924097 @default.
• W2070284483 date "2004-01-01" @default.
• W2070284483 modified "2023-10-10" @default.
• W2070284483 title "Purification and properties of the<i>Escherichia coli</i>nucleoside transporter NupG, a paradigm for a major facilitator transporter sub-family" @default.
• W2070284483 cites W1043088292 @default.
• W2070284483 cites W1526754730 @default.
• W2070284483 cites W1604161893 @default.
• W2070284483 cites W1649674936 @default.
• W2070284483 cites W1778382208 @default.
• W2070284483 cites W1967675162 @default.
• W2070284483 cites W1985420286 @default.
• W2070284483 cites W2007376743 @default.
• W2070284483 cites W2014550271 @default.
• W2070284483 cites W2029169337 @default.
• W2070284483 cites W2034092705 @default.
• W2070284483 cites W2045684566 @default.
• W2070284483 cites W2053765655 @default.
• W2070284483 cites W2061148513 @default.
• W2070284483 cites W2068004788 @default.
• W2070284483 cites W2069573006 @default.
• W2070284483 cites W2074656054 @default.
• W2070284483 cites W2082381093 @default.
• W2070284483 cites W2097382368 @default.
• W2070284483 cites W2099220940 @default.
• W2070284483 cites W2099384461 @default.
• W2070284483 cites W2101625991 @default.
• W2070284483 cites W2102264945 @default.
• W2070284483 cites W2119651028 @default.
• W2070284483 cites W2128797367 @default.
• W2070284483 cites W2130381741 @default.
• W2070284483 cites W2133792240 @default.
• W2070284483 cites W2140238053 @default.
• W2070284483 cites W2147354493 @default.
• W2070284483 cites W2152423377 @default.
• W2070284483 cites W2152517415 @default.
• W2070284483 cites W2157787228 @default.
• W2070284483 cites W2158714788 @default.
• W2070284483 cites W2163282166 @default.
• W2070284483 cites W2164248708 @default.
• W2070284483 cites W2410120918 @default.
• W2070284483 cites W3110343827 @default.
• W2070284483 cites W4244804481 @default.
• W2070284483 doi "https://doi.org/10.1080/09687860400003941" @default.
• W2070284483 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15513740" @default.
• W2070284483 hasPublicationYear "2004" @default.
• W2070284483 type Work @default.
• W2070284483 sameAs 2070284483 @default.
• W2070284483 citedByCount "48" @default.
• W2070284483 countsByYear W20702844832012 @default.
• W2070284483 countsByYear W20702844832013 @default.
• W2070284483 countsByYear W20702844832014 @default.
• W2070284483 countsByYear W20702844832015 @default.
• W2070284483 countsByYear W20702844832016 @default.
• W2070284483 countsByYear W20702844832017 @default.
• W2070284483 countsByYear W20702844832018 @default.
• W2070284483 countsByYear W20702844832019 @default.
• W2070284483 countsByYear W20702844832020 @default.
• W2070284483 countsByYear W20702844832021 @default.
• W2070284483 countsByYear W20702844832022 @default.
• W2070284483 countsByYear W20702844832023 @default.
• W2070284483 crossrefType "journal-article" @default.
• W2070284483 hasAuthorship W2070284483A5004045461 @default.
• W2070284483 hasAuthorship W2070284483A5009942080 @default.
• W2070284483 hasAuthorship W2070284483A5012010132 @default.
• W2070284483 hasAuthorship W2070284483A5015381194 @default.
• W2070284483 hasAuthorship W2070284483A5023251665 @default.
• W2070284483 hasAuthorship W2070284483A5027067788 @default.
• W2070284483 hasAuthorship W2070284483A5034487444 @default.
• W2070284483 hasAuthorship W2070284483A5037331354 @default.
• W2070284483 hasAuthorship W2070284483A5055099992 @default.
• W2070284483 hasAuthorship W2070284483A5060865138 @default.
• W2070284483 hasAuthorship W2070284483A5076361697 @default.
• W2070284483 hasAuthorship W2070284483A5078924097 @default.
• W2070284483 hasBestOaLocation W20702844831 @default.
• W2070284483 hasConcept C104317684 @default.
• W2070284483 hasConcept C115335371 @default.
• W2070284483 hasConcept C12554922 @default.
• W2070284483 hasConcept C133571119 @default.
• W2070284483 hasConcept C149011108 @default.
• W2070284483 hasConcept C185592680 @default.
• W2070284483 hasConcept C2776543447 @default.
• W2070284483 hasConcept C2780549722 @default.
• W2070284483 hasConcept C41625074 @default.
• W2070284483 hasConcept C547475151 @default.
• W2070284483 hasConcept C55493867 @default.

• next