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- W2070291173 abstract "ProNectin F (PnF) was chemically modified by introducing some functional groups to prepare various derivatives of primary amino (PnF-N1), tertiary amino (PnF-N3), quaternary ammonium (PnF-N4), carboxyl (PnF-COOH) and sulfonyl groups (PnF-SO3H). When C3H10T1/2 cells were cultured on non-treated dishes coated with the derivatives, the number of mesenchymal cells attached to the culture dishes increased for the coating with PnF-COOH and PnF-SO3H, even at their low adsorption amount. The cytotoxicity was high for the coating of PnF-N1 and PnF-N4 compared with that of the PnF-N3, PnF-COOH and PnF-SO3H. The treatment with integrin α5 and αV antibodies suppressed the cell attachment to the dishes coated with PnF-COOH and PnF-SO3H. The phosphorylation of extracellular signal-regulated kinase (ERK) was upregulated for cells attached to the dishes coated with PnF-COOH and PnF-SO3H, indicating their enhanced proliferation. It is concluded that the chemical derivatization of PnF enhanced the ability of cell attachment and proliferation." @default.
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- W2070291173 date "2013-02-01" @default.
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- W2070291173 title "Effect of ProNectin F derivatives on cell attachment and proliferation" @default.
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- W2070291173 doi "https://doi.org/10.1016/j.actbio.2012.07.039" @default.
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